研究表明丝氨酸蛋白酶抑制因子通过控制内源性或外源性蛋白酶的蛋白水解活性，参与生物体内广泛而重要的生理活动。在寄生虫感染过程中，丝氨酸蛋白酶抑制因子是逃逸宿主免疫反应的重要因子，在抑制宿主的防御功能中发挥重要作用。因此研究其免疫调节机制如抑制抗原加工、细胞凋亡和免疫细胞分泌的丝氨酸蛋白酶的功能，将对寄生虫病的防治具有重要的意义。本研究拟采用体外表达及放射性标记等方法对我们之前免疫筛选得到的旋毛虫丝氨酸蛋白酶抑制因子(Ts-serpin)与宿主免疫细胞分泌的不同类型的丝氨酸蛋白酶的相互作用进行分析，并利用分子生物学和免疫学方法对其抑制细胞毒性T细胞诱导的细胞凋亡以及对树突状细胞和巨噬细胞功能的影响进行探究，从而为旋毛虫与宿主间的互作研究提供参考，同时为揭示旋毛虫及相关寄生虫的免疫逃逸机制提供新的理论依据。

Serine protease inhibitors play a variety of important biological roles by controlling endogenous and exogenous proteolytic activities. During parasite infection, serine protease inhibitors are important factors in escaping host immune response and play important roles in suppressing the host defense function. It is greatly signification for preventing parasite diseases to study the immunomodulatory mechanism of serine protease inhibitors, such as inhibition of antigen processing, apoptosis and function of the serine protease derived from host immune cell. In this study, we analyze the interaction between Trichinella spiralis serine protease inhibitor (Ts-serpin) obtained previously by immune screening and the different serine protease derived from host immune cell by transcription-translation in vitro and radiolabeled method. Furthermore, we explore immune regulation roles of Ts-serpin in suppressing cytotoxic T-lymphocytes cells induced apoptosis and regulation of activity of dendritic cell and macrophage by molecular biology and immunological methods, which could provide reference for understanding interaction between Trichinella spiralis and host. Meanwhile, it will also provide a new theoretical basis for the immune escape mechanisms of Trichinella spiralis and other related parasites.