宫颈癌是新疆特高发疾病之一。前期工作中，通过维吾尔族妇女宫颈癌的血清蛋白质组学、代谢组学和表观遗传学等多方面研究，我们从系统生物学水平初步认识宫颈癌发病机制和早期预警指标。但是，很多问题直接涉及宫颈癌组织细胞内蛋白质表达调控网络，而与此相关的文献甚少。人乳头瘤病毒（human papillomavirus, HPV）感染，尤其是高危型HPV16持续感染可能是宫颈癌发生的主要原因，但是病毒感染与癌前病变的潜伏期较长，HPV筛查预警潜力极其有限。故本课题拟利用维吾尔族妇女宫颈癌疾病资源,依托二维液相色谱、双向荧光差异电泳和质谱等技术平台，通过HPV16阳性宫颈病变组织的蛋白质组学分析及候选差异表达蛋白的发现和定量鉴定，探讨HPV持续感染及宫颈癌特异性改变的共性蛋白质表达调控网络，了解宫颈癌发病机制的肿瘤共性和族群特点，建立基于HPV筛查的宫颈癌预警辅助诊断指标，提高新疆宫颈癌防治水平，并促进多学科发展。

Cervical cancer has been one the high-incident diseases in Xinjiang. In previous works, we have preliminarily understood the mechanisms and early prognostic markers of cervical cancer in Uighur women at systems biology level by a series of studies including serum proteomics, metabonomics, and epigenetics. However, many questions arised about the cellular regulation network of protein expression in cervical cancer tissues, which was poorly described in the literature. The infection by human papillomaviruses (HPV) especially the persistent infection by high-risk type HPV16 may be the main etiological factor responsibe for cervical carcinogenesis, but there is a relatively long latent period for cervical precursor development after virus infection, thus HPV screening has a very restrictive potential in cancer prognosis.In this project, we will focus on Uighur women with cervical carcinoma and analyze HPV16-positive cervical lesions and identify candidate differentially expressed proteins followed by quantitative detection, by a proteomics approach using technological platforms including two-dimensional liquid-phase chromatography, two-directional fluorescence in-gel electrophoresis and mass spectrometry etc., study the regulation network of protein expression altered by, and specific to, persistent HPV infection and cervical carcinogenesis,for understanding the mechanisms of cervical carcinogenesis common to tumors and the characteristics unique to the ethnic population，and establishing an auxiliary diagnostic profile for early prognosis of cervical cancer in addition to HPV detection.This study is important to improve the management quality of cervical cancer prevention and treatment in Xinjiang, and promote multidisciplinary development as well.