泡状棘球蚴病是一种严重危害人体健康、被我国列为重点防治的人兽共患病。目前对该病的致病机理、宿主应激反应以及宿主-寄生虫相互作用的调控网络机制尚未完全阐明，同时亟需发展该病的新型免疫诊断技术及有效治疗手段。我们前期工作中建立了蛋白质组学技术及数据分析信息平台，对宿主肝脏基因表达变化进行初步研究，发现部分代谢相关蛋白的表达下调。本项目中我们将运用基因芯片技术和蛋白质组学技术，结合生物信息学分析手段，从基因转录及翻译水平对泡状棘球蚴感染中晚期后宿主肝脏基因表达变化进行高通量分析研究。通过分析表达变化极为显著的基因/蛋白，鉴定出宿主肝脏被影响的一系列生物学功能及相关信号转导通路，筛选可用于泡状棘球蚴病诊断、治疗、病情监测、疗效评估的候选分子，以及肝脏代谢病理损伤的特异性标记分子。本项目对加深探索泡状棘球蚴致病机理及宿主病理变化的分子机制具有重要意义，为发展该病的新型诊疗技术提供理论基础和技术支持。

Human alveolar echinococcosis (AE) is one of the most severe zoonosis. At the present time, the molecular mechanism of pathogenesis, host immune response and parasite-host relationship has not been fully illustrated. As such, the new therapeutic methods and effective diagnostic techniques are needed to be improved. In previous study, we established technology platform for proteomics and mass data analysis. We also investigated host hepatic gene expression and found that most of the down-regulated proteins are related to metabolism. In this project, we will conduct a high-throughput analysis using microarray and proteomic technology to investigate the host hepatic gene/protein expression profile. We will identify a series of biological function and signaling pathways in response to AE. Based on the analysis of genes with significantly changed expression, specific molecular bio-markers would be identified and characterized for diagnosis, drug targeting therapy, evaluation of therapeutic effects and control of AE. This study will be helpful for understanding the molecular mechanism of pathogenesis, as well for developing the new therapeutic and diagnostic methods for AE.