吲哚是一种常见的氮杂环芳香化合物，在微生物生理生化及人类疾病与健康等方面扮演着重要的角色，而高浓度的吲哚对生物具有一定毒性，危害生态环境和人类健康。吲哚的生物转化研究是阐明其在复杂体系中生物学意义的重要组成部分，也是吲哚污染修复的关键。虽然吲哚转化研究已持续近百年，但仍存在降解菌株资源缺乏、降解途径和分子机制不明晰等研究现状。本项目以实验室前期获得的高效吲哚降解菌Burkholderia sp. IDO3为研究对象，将详细解析菌株IDO3降解吲哚的中间产物，推测其降解途径。进一步通过对菌株进行基因组和转录组测序分析，定位参与吲哚代谢的关键功能基因(簇)。利用基因突变、异源表达和酶学特性研究阐明菌株IDO3中负责吲哚加氧、靛红水解和邻氨基苯甲酸代谢的功能基因及其特性。本项目旨在揭示Burkholderia菌属降解吲哚的途径及关键基因，补充和完善吲哚分子代谢机制，促进其生物学功能及修复研究。

Indole is a common N-heterocyclic aromatic compound, which plays an important role in the microbial physiological and biochemical properties, and human diseases and health. High concentrations of indole have certain toxicity to organisms and should endanger the ecological environment and human health. Indole biotransformation study is an important part to clarify its biological significance in a complex system, and it is also the key for indole pollution remediation. Although the study of indole transformation has lasted for nearly a century, the bacterial resources for indole degradation are still lacking, and the degradation pathways and molecular mechanisms remain unclear. In this project, Burkholderia sp. IDO3, a novel and highly efficient indole-degrading bacterium previously obtained in our laboratory, is used as the research object. The degradation product and chemical degradation pathway of indole in strain IDO3 will be determined in detail. Then the functional genes (clusters) involved in indole metabolism are mapped by genome and transcriptome sequencing. Finally, the functional genes responsible for indole oxidation, isatin hydrolysis and anthranilic acid metabolism will be elucidated in-depth by gene mutation, heterologous expression and enzymatic property studies. This project aims to reveal the degradation pathway and key functional genes of indole degradation in Burkholderia strains, which will perfect the indole molecular metabolic mechanism and promote its biological function and remediation studies.