细胞质雄性不育"三系"是大豆杂交种选育的基础材料。其中，强恢复系的选育非常繁琐，且恢复力只能通过与不育系的测交来鉴定，既耗时又费力。本研究以配子体遗传的RN型细胞质雄性不育系与其导入强恢复基因的近等基因系杂交获得的F2代分离群体为材料，通过单株育性鉴定,明确恢复基因遗传模式；再利用高密度SSR标记，定位恢复基因Rf1。然后，利用可育和不育DNA文库分别与Rf1基因定位区间特异序列捕获芯片杂交，对杂交片段进行重测序，开发该区间SNPs和InDels标记，进行多态性检测，精细定位Rf1基因。最后，参照大豆基因组测序和重测序数据库，结合其它植物已克隆的Rf基因特征，对精细定位区段候选基因进行筛选分析，并初步鉴定候选Rf1基因。通过本研究所获得的连锁标记，有望加速RN型恢复系的选育进程；同时，筛选鉴定的候选基因，也将为今后克隆Rf1基因和解析RN型细胞质雄性不育育性恢复机制奠定基础。

Cytoplasmic male sterility "three-lines" are the basic materials for soybean hybrid breeding. Breeding for strong restorer lines is very complicated and restoring ability can only be identified by testcross with male sterile lines, which is time-consuming and labor-intensive. F2 segregating population, which was obtained by crossing between the gametophyte genetically RN-cytoplasmic male sterile line and its near-isogenic line with strong restorer gene, will be used for the Rf1 gene mapping through determination of the inheritance pattern by sterility identification of each generation and further polymorphism analysis by high density of SSR markers. Then for fine mapping of the Rf1 gene, SNPs and InDels will be developed by target sequence capture microarray and re-sequencing of restorer gene location region of the fertile and sterile DNA library, respectively. Finally, candidate Rf1 gene will be screened in the fine mapping region based on the soybean genome sequencing and re-sequencing database,combined with other plants Rf gene characteristic. In conclusion, the linked markers Rf1 identified in this study will accelerate the breeding process of RN-restorer lines, meanwhile the identified candidate genes will lay the foundation for Rf1 gene cloning and RN-CMS fertility restoration mechanism exploration in future.