目前植物-土壤微生物反馈假说阐释外来植物成功入侵的研究多将土壤微生物群落作为灰箱甚至黑箱；且随着全球变暖和氮沉降加剧，外来植物成功入侵的土壤微生态学机制愈加复杂。本项目基于新一代宏基因组学理论和技术对入侵植物不同入侵程度、外来非入侵近缘植物和本地近缘种不同盖度的土壤微生物群落结构进行全景式对比分析，以验证我们提出的外来植物成功入侵-土壤微生物群落共演化假说：外来植物在成功入侵进程中改变了原有土壤微生物群落结构和功能；土壤微生物群落重构更利于入侵植物的生长扩张；土壤微生物群落的持续演替将越利于该入侵植物，进而达到最利于有该入侵植物生长的某种稳态；全球变暖和氮沉降均促进该共演化，且两者具有叠加效应。研究结果不仅在理论上进一步佐证外来植物成功入侵的土壤-微生物反馈假说，阐明全球变暖和氮沉降下外来植物成功入侵的土壤微生态学机制，丰富入侵生态理论体系；且在实践上也为入侵植物的防控提供切实的理论指导。

The plant-soil feedback (PSFs) hypothesis is often invoked to explain successful invasion of exotic plants. However, existing studies on PSFs mainly regard the invaded soil microbial community as a grey box, or even a black box. Thus, the increased global warming and nitrogen deposition might make it more complex in the soil micro-ecological mechanisms of exotic plant invasion. The present project will be carried out using the new-generation metagenomics methods and technology (i.e., combination of metagenomics and new-generation high throughput sequencing technology) to gain insights into the community structure and metabolic characteristics of soil microorganisms under different degrees of invasion mediated by the notorious invasive plants (i.e., Conyza canadensis), and different coverage of exotic non-invasive (i.e., Cosmos bipinnata) and native species (i.e., Pterocypsela laciniata) in China. This project aims to validate the following co-succession hypothesis: (1) exotic invasive plants could drive the succession of community structure and metabolic characteristics of soil microorganisms during their invasion process; (2) the re-construction of community structure and metabolic characteristics of soil microorganisms mediated by exotic invasive plants is beneficial for their own growth and expansion; (3) the succession of community structure and metabolic characteristics of soil microorganisms increased obviously with the enhanced invasion degree of exotic invasive plants, which is more conducive to the growth and expansion of exotic invasive plants, finally, it will reach a stable state in which soil microbial community can make the best of facilitating the further invasion of exotic plants; and (4) global warming and nitrogen deposition could accelerate the co-succession process between exotic plant invasion and soil microbial community, and both they have synergistic effects on the co-succession process between exotic plant invasion and soil microbial community. These studies will not only theoretically provide evidence for the PSFs hypothesis, and enrich the system of invasion ecology, especially the soil micro-ecological mechanisms of exotic plant invasion, but also in practice lay an important theoretical foundation for effective invasion prevention and control under global warming and nitrogen deposition.