年龄途径在植物成花过程中发挥着非常重要的调控作用。竹类植物具有特殊的开花习性，目前对其成花机理研究非常有限。为揭示年龄途径在竹类植物成花诱导中的调控机制，本项目拟利用高通量测序、植物转基因等技术结合分子生物学手段，重点分析翠竹miR156及其关键靶基因SPLs参与成花转变的生物学功能。首先明确miR156及开花相关SPLs在开花和未开花翠竹中的相对表达量；其次利用mir156拟南芥突变体进行翠竹miR156基因的功能互补验证；同时培育miR156基因抑制表达转基因翠竹，定量分析miR156及其关键靶基因SPLs在转基因株系中的相对表达水平，并进一步验证它们之间的互作关系。通过以上研究探究翠竹成花过程中miR156-SPLs年龄途径的分子机理，丰富竹类植物生殖生物学的研究基础，为竹类植物成花转变相关研究提供理论依据。

Age pathway plays an important regulatory role in plant transformation from vegetative growth to reproductive growth. At present, the study on molecular mechanism of floral transition is very limited in bamboo plants as their special flowering habits. To reveal regulatory mechanism of age pathway in floral induction of bamboo plants, the study focused on biological function analysis of miR156 and its key target SPLs by high-throughput sequencing, plant transgenic technology etc., combined with molecular biological methods. Firstly, to analyze the relative expression pattern of miR156 and flowering-related SPLs in flowering and non-flowering Pleioblastus pygmaeus; Secondly, to verify functional complementation role of miR156 gene of Pleioblastus pygmaeus in mir156 Arabidopsis mutants; Meanwhile, to obtain transgenic Pleioblastus pygmaeus suppressing miR156, and to quantify the relative expression level of miR156 and its key target SPLs in transgenic plants; Finally, to verify the interaction relationship between miR156 and its key target SPLs. The study aims to explore molecular mechanism of miR156 regulating its key target SPLs in flowering age pathway of Pleioblastus pygmaeus, which will enrich the research of reproductive biology in bamboo plants and provide theoretical basis for the analysis of floral transition in bamboo plants.