生殖细胞的正常发育是显花植物世代交替的基本前提，也是农业生产应有的保证。有丝分裂是生殖细胞(雌雄配子体)发育成熟的必经过程之一，而与有丝分裂过程相关的基因表达调控目前还知之甚少。我们筛选到一株雌雄配子体严重受到影响的突变体mt190，初步的分析结果表明雌配子体异常于功能大孢子的第一次有丝分裂，雄配子体发育止于小孢子的第一次有丝分裂。通过PCR和相关的生物信息学软件分析，在MT190基因的3'-UTR区有一段miR838结合序列，暗示miR838可能负调控MT190基因的表达而控制配子体细胞的第一次有比分裂过程。本项目拟利用各种细胞生物学、遗传学和生物化学实验手段揭示MT190基因在拟南芥生殖细胞早期发育的有丝分裂过程中的功能。

The development of germ cells is a basic premise in generational alternation in flowering plants, also, it is a kind of guarantee on agricultural production. Mitosis is one of necessary processes for germ cells(male and female gametophyte) development and maturation. But as far, the regulation of gene expression during mitosis is still poorly understood. We screened a male and female gametophyte seriously affected mutant mt190 and preliminary analysis of results showed that the female gametophyte abnormal in the functional megaspore at the first mitosis and male gametophyte development stopped at the same process. Analysis by PCR and bioinformatic softwares, The 3'-UTR of MT190 gene has miR838 binding sequence, suggesting that miR838 maybe negative regulate MT190 gene expression and probably control gametophyte cells at the first mitosis. The project intends to use a variety of cell biology, genetics and biochemistry experimental means to reveal the function of the MT190 genes at mitosis in Arabidopsis early development of germ cells.