天然产物纯化用稀土磁性纳米材料研究

天然产物因其结构多样性和强的生物活性，一直是新药研发的高质量化合物库。但成分的复杂性，其分离纯化一直是天然药物研究面临的关键难题，最关键的问题是缺乏化合物的结构选择性分离方法。磁性纳米吸附材料具有其独特优势，稀土元素因其特殊的配位能力，在结构识别上有着极强的优势。稀土基磁性亲和材料已在蛋白质组学研究中实现了质谱检测前对磷酸化肽的高选择性捕获、快速磁分离提纯和方便的磷酸化肽质谱标记。.本研究拟以黄酮、生物碱、皂苷等为模型化合物，研究、考察稀土离子和稀土氧化物对目标化合物的配位特性（结构选择性、动力学、溶剂环境）；合成基于稀土配位的磁性纳米粒子，研究粒子对天然产物的吸附选择性、容量、解吸附条件等，以不同模式植物：黄芩（富含黄酮）、黄连（富含生物碱）、人参（富含皂苷）研究选择性吸附情况，采用液相色谱-串联质谱法判别吸附的结构选择性。研究成果为天然产物的制备分离提供高选择性的预纯化方法。

Natural products have been a high quality compound library for the development of new drugs due to their structural diversity and strong biological activity. However, the separation and purification of the components is a key problem in the research of natural medicine. The most important problem is the lack of the selective separation of the compounds. Magnetic nano materials have their unique advantages, and rare earth elements have a strong advantage in the structure recognition because of their special coordination ability. The rare earth based magnetic affinity materials have been used for the study of proteomics, which can achieve high selective capture, rapid magnetic separation and purification of peptide and mass spectrometric analysis..This study intends to study the coordination properties of rare earth ions and rare earth oxides on the of target natural products (structural selectivity, dynamics and solvent environment) using flavonoids, alkaloids, saponins as model compounds, and synthesis of rare earth magnetic nanoparticles with selective adsorption ability to natural products. Using Scutellaria baicalensis (rich in flavonoids), Coptis chinensis (rich in alkaloids), ginseng (rich in saponin) as model plants, the adsorption selectivity of nano materials will be investigated by tandem mass spectrometry. The research results will provide a novel highly selective pre-purification method for the separation and preparation of natural products.