基于植物色素提取的双水相气浮溶剂浮选体系的构建及分离行为和机理研究

本课题旨构建水溶性小分子有机溶剂-盐双水相体系与气浮溶剂浮选耦合的绿色提取新方法：双水相气浮溶剂浮选(Aqueous Two-phase Solvent Flotation, ATPSF)，即建立一种以廉价、安全、无毒的分相剂分相，气-液选择性分离，液-液选择性相转移的高选择性、高富集倍数、绿色无污染的新型分离/富集体系。本课题拟选择叶绿素与花青素为目标物，利用双水相体系自身的组成特点，综合运用有机溶剂法及盐法对植物细胞进行破壁；筛选出适合叶绿素及花青素分离纯化的分相体系；进行优化实验，探讨各个因素的影响机制；研究双水相气浮溶剂浮选体系的分相、相转移机制；建立适用于双水相气浮溶剂浮选体系中叶绿素和花青素分离纯化的热力学和动力学模型；并进行新方法的实效性考察，实现植物色素的绿色提取，降低生产成本，提高提取效率和产品质量，为医药、生物及食品等产业中生物活性功能因子的提取提供一种新的方法。

The purpose of this topic is to construct a new coupling green separation method between small molecular water-soluble organic solvent-salt aqueous two phase systems (ATPS) and solvent sublation (SS): aqueous two-phase solvent flotation (ATPSF). That is to construct a new separation/enrichment system has the advantages of popular price, safe, green, non-toxic, high selectivity and high enrichment multiples. This topic aim to select chlorophyll and cyanidin as the targets; take the advantages of the compositional characteristics of ATPS itself to make plant cells broken by comprehensively utilizing solvent and salt; screen the appropriate ATPS for the separation of chlorophyll and cyanidin; carry out the optimizing experiments; discuss the influence mechanisms of the different factors; study the mechanisms of phase-separation and phase-transfer; construct the thermodynamic and dynamic models for the separation of chlorophyll and cyanidin in the ATPSF systems; study on the actual effect of the new method. The topic it to bring about green separation of the plant pigments, reduce production cost, and provide a new method for the separation of biologically active factors in the food, medical and biological industries.