基于苯硼酸基元的亲和温敏聚合物双水相浮选体系的构建及其可控选择性分离纯化多糖研究

多糖提取液基质复杂，如何优化分离纯化方法，在提高多糖产率和纯度的同时，保持其生物活性，是推动多糖应用开发的一个亟待解决的问题。本课题拟构建苯硼酸基元亲和温敏聚合物双水相浮选体系，用于多糖可控选择性分离纯化。主要研究内容及目标：①制备新型温敏成相聚合物，丰富现有温敏聚合物双水相体系，解决目前体系构建受限于商品化品种少，难以满足多样化应用需求的问题；②将温敏聚合物双水相体系与传统溶剂浮选耦合，构建温敏聚合物双水相浮选体系，解决双水相萃取受相比制约、富集倍数有限，以及传统溶剂浮选环境污染问题；③设计合成含苯硼酸基元的两亲性温敏嵌段共聚物作为捕集剂，构建苯硼酸基元亲和温敏聚合物双水相浮选体系强化对多糖分离的选择性，同时，基于捕集剂的温度响应行为，实现多糖控温分级沉淀，解决目前浮选药剂难以分离回收和选择性不佳的问题。深入分析并解决相关科学问题，使新方法能应用于复杂基质中活性组分的大规模分离纯化。

The component of polysaccharide extracts is complex, so how to construct an effective separation and purification method is an urgent issue which promotes the application and development of polysaccharide. That is to construct a new method has the advantages of high yield, high purity and biocompatibility for polysaccharide. This topic is to construct a phenylboronic acid moieties based affinity thermosensitive polymer aqueous two-phase flotation system (ATPFS) for the controlled selective separation and purification of polysaccharide. The main research contents and targets are as follows: ①Prepare new thermosensitive phase-separation polymers to enrich the existing thermosensitive polymer aqueous two-phase systems(ATPS). The aim is to resolve the following problems: the construction of thermosensitive polymer ATPS is limited by less commercialized varieties of thermosensitive polymer, which is difficult to meet diversified application requirements. ② Construct the thermosensitive polymer ATPFS coupling the thermosensitive polymer ATPS and solvent sublation (SS). The aim is to resolve the following problems: the enrichment coefficient of aqueous two-phase extraction (ATPE) is limited as a consequence of constrained phase ratio of ATPS, and the environmental pollution problem of traditional SS. ③Design and synthesize amphipathic thermosensitive block copolymers with phenylboronic acid moieties as trapping agents, and construct a phenylboronic acid moieties based affinity thermosensitive polymer ATPFS to improve separation selectivity for polysaccharide, meanwhile, implement temperature controlled fractional precipitation based on temperature response behavior of trapping agents. The aim is to resolve the following problems: the difficulty in separation and recovery of trapping agent, and the poor selectivity. The related scientific problems of this topic will be deeply analyzed and resolved, and it will make this new method be applied to large-scale separation and purification of active components in complex systems.