项目研究脉冲电场干预下杠杆定位手法对腰椎间盘的生物力学特性及对其神经反馈机制的影响，探讨电场和杠杆定位手法临床疗效的相关性，为临床应用提供理论依据。研究以家兔为对象，分6种脉冲电场强度实验组，用自身对照法依次给予手法、脉冲电场结合手法、腰椎损伤模型下的手法、脉冲电场+手法等操作，研究脉冲电场结合杠杆定位手法对腰椎间盘的生物力学特性以及神经反馈机制。通过生物力学数据采集分析系统同步采集杠杆定位手法力、应变、肌电图，建立有限元模型，用神经网络算法求解生物力学参数，阐明电场与杠杆定位手法对腰椎间盘的生物力学特性和神经反馈机制的影响，同时对健康志愿者进行人体测试验证。本研究主要基于杠杆定位手法结合电磁经络通能提高治疗腰椎间盘突出症有效率的临床新发现，探索脉冲电场与杠杆定位手法联合作用下的腰椎间盘生物力学特性的变化规律，为腰椎间盘突出症的多方法治疗作用机制的系统研究提供科学依据。

Objective:To study the affection of the biomechanicsfeatures and the neural feedback mechanismand with the lever positioning manipulation(LPM) on the lumbar disc under the intervention of the pulse electric field(PEF).To divide into six groups of rabbits for different interventions of the PEF. Each group will be tested on following sequence: using LMP only, LMP+PEF, LMP after lumbar damaged only and LMP+PEF after lumbar damaged. We study the affection of the biomechanicsfeatures and the neural feedback mechanismwith the intervention of the PEF and the LPM on the lumbar disc by self-control method.To build the biomechanics model of the lumbar disc, to collect the data of stress, strain and electromyography and build the biomechanics finite element model ,to clarify the electric field and lever positioning technique on the biomechanical characteristics of the lumbar and nervous feedback mechanismon the basis ofbiomechanical parameters with neural network algorithm, and to carry on test validation of the healthy volunteers.This study is based on the new fact that the LMP combined with electromagnetic meridian-therapeutic to treat the LDH performs better than the independent electromagnetic meridian-therapeutic treatment. Searching for the affection of thebiomechanicsfeaturesupon the experiment of the LMPcombined withthe PEF can provides the scientific evidence ofsystematic researchof Multi-Method Therapy on LDH.