在我们多年来化学发光分析及生物传感研究基础上，借鉴前人有关DNA纳米技术和脱氧核酶的研究成果，本项目拟以DNA的生物属性和氯化血红素（Hemin）的催化性能为基础，以DNA-Hemin复合物为构筑单元，利用DNA结构可设计性进行精确组装，得到结构丰富的DNA-Hemin组装体，系统研究各种组装体的催化性能，揭示组装结构与催化活性之间的规律，探索催化型DNA-Hemin分子开关，以化学发光为检测信号构建高效的生物传感新方法。本项目将DNA与Hemin共价结合，既保留了DNA卓越的分子识别功能，又引入了Hemin高效的信号转导功能，实现了识别与信号转导功能的一体化，为生物传感的设计提供一种新思路。这类新型传感既具有DNA纳米技术在分子识别方面的优势，又具有化学发光法在检测方法的优势，为临床医学、食品安全等领域提供新的检测方法。同时，本项目也将丰富脱氧核酶的研究内容，推动化学发光研究向纵深发展。

Because of their exquisite recognition properties encoded in the specific interaction of Watson-Crick base-pairing, deoxyribonucleic acids (DNAs) can be programmed and assemble with each other in a predictable manner. In this proposal, we aim to accurately assemble the covalent DNA-Hemin adduct through DNA nanotechnology and suggest new chemiluminescence biosensing strategy. DNA-Hemin adduct will be used as building block to build many nanostructured materials. The DNA-Hemin assembly will be used as the chemiluminescence catalyst to investigate the catalytic activity in detail. At the same time, the structures of the DNA-Hemin assembly will be characterized with some techniques (such as FT-infrared spectra and CD spectra). The relationship between catalytic activity and the structure of DNA-Hemin assembly will be explored. Then, we will make full use of the structure-activity relationship of DNA-Hemin assembly to suggest some new strategies for chemiluminescence biosensing. Furthermore, some signal amplification methods will be introduced into the method to improve the sensitivity. This new biosensing method has the advantages of DNA nano technology in molecular recognition, and also has the advantage of chemiluminescence detection. The features make the proposed assay particularly useful in clinical biochemistry laboratory settings. Therefore, the proposal not only will open a new research area for chemiluminescence analysis, but also will be helpful for us to know the activity of peroxidase-mimicking DNAzyme.