人类卵泡液中游离microRNA：寻找与胚胎发育潜能相关的新的分子标记物及研究相应分子机制

辅助生殖技术使得患者一次成功受孕率仅为40%左右。其中影响妊娠率的重要的因素之一便是胚胎质量。临床上，目前只能通过形态学评分来判断卵子级别及胚胎质量优劣。但是通过形态学的指标往往很难反映胚胎真实的发育潜能及植入结局。.近期，国际上发现有大量miRNAs存在于人类卵泡液中。卵泡液miRNAs是否能够成为预测胚胎质量的新的分子指标还未被报道。因此，是否能够找到卵泡液中特定的miRNAs与胚胎发育潜能相关，并解析其中的分子机制，正是本课题所要聚焦的重要科学问题。本课题拟采用ICSI患者初始单个卵泡的卵泡液为研究样本，对从其中分离的卵子授精后囊胚质量及临床妊娠情况进行跟踪、评价及分组，发现并在独立样本中验证差异miRNAs。最后以鼠MII期卵子为模型，揭示出与胚胎发育潜能相关的卵泡液miRNAs及相应分子机制。最终，为以卵泡液成份为研究对象，进行人类胚胎发育潜能的临床研究提供新角度及新思路。

The successful pregnant rates for IVF is only about 40%. Embryonic developmental potentials is one of the most important factors for successful pregnancy. In clinical, oocyte and embryo quality can only be demonstrated by morphology scoring. But the morphology can hardly indicate real oocyte and embryonic developmental potentials..Recently, a few microRNAs have been found in human follicular fluids. It is unknown that whether follicular microRNAs can be regarded as the new predictor of human embryonic developmental potentials. In this proposal, we focus on the following important question: What are roles and mechanism of microRNAs in follicular fluids in affecting embryo quality? We are planning to acquire follicular fluids from the first follicle containing an oocyte. The development of oocytes are monitored and scored. The follicular fluids are divided into groups based on the development scoring and clinical outcome. The differential expression of microRNAs level of follicular fluids between groups is investigated. Finally, the function of corresponding differentiated microRNAs is studied in mouse oocytes. The findings will figure out novel role of follicular fluids microRNAs in embryo developmental potential and provide insights for human IVF clinics.