Integrins β1 and β3 are biomarkers of uterine condition for embryo transfer.
Integrins β1 and β3 are biomarkers of uterine condition for embryo transfer.
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整合素β1和β3是胚胎移植子宫状况的生物标志物。
DOI：
10.1186/s12967-016-1052-0
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发表时间：
2016-10-26
影响因子：
7.4
通讯作者：
Sun X
中科院分区：
文献类型：
--
作者：
Chen G;Xin A;Liu Y;Shi C;Chen J;Tang X;Chen Y;Yu M;Peng X;Li L;Sun X
临床促排卵会导致血液雌激素 (E2) 超过生理水平，从而阻碍子宫容受性。相反，黄体酮会产生相反的临床效果，这表明它可能能够恢复因暴露于高雌激素水平而失去的接受能力。整合素是监测子宫状况最广泛使用的生物标志物。我们研究了黄体酮诱导的整合素β表达模式的变化，作为子宫容受性随雌激素水平增加而变化的生物标志物。对患者的子宫内膜活检样本进行雌激素 (E2) 和孕激素 (P4) 含量以及整合素 β1 和 β3 表达水平的筛查。在胚胎附着模型中使用人子宫内膜腺癌细胞评估子宫容受性。评估了胚胎附着的各自和连锁效应以及腺癌细胞质膜上整合素 β1 和 β3 表达模式的变化对 100 nM 浓度的 E2 和 P4 的反应。血液 E2 浓度升高与子宫活检样本中整合素 β3 表达水平显着降低相关。体外实验表明，100 nM E2 浓度抑制胚胎附着模型中使用的人子宫内膜腺癌细胞质膜上整合素 β3 的分布，并导致胚胎附着率降低。相反，P4增强了整合素β1的表达并促进其在质膜上的分布。此外，P4 恢复了因暴露于 100 nM E2 而损失的胚胎附着效率。血液E2和P4水平以及子宫活检样本中整合素β3和β1表达水平应被视为评估子宫容受性和确定胚胎移植最佳时间的生物标志物。 试用注册 试用号：ChiCTR-TRC-13003777；注册中心名称：中国临床试验注册中心；注册日期：2013年9月4日；第一位研究参与者的注册日期：2013 年 10 月 15 日 本文的在线版本 (doi:10.1186/s12967-016-1052-0) 包含补充材料，可供授权用户使用。
Clinical ovulation induction induces blood estrogen (E2) in excess of physiological levels, which can hinder uterine receptivity. In contrast, progesterone produces the opposite clinical effect, suggesting that it might be capable of recovering the lost receptivity resulting from exposure to high estrogen levels. Integrins are the most widely used biological markers for monitoring uterine conditions. We studied progesterone-induced changes in integrin β expression patterns as biomarkers for changes in uterine receptivity in response to increased estrogen levels. Endometrial biopsy samples from patients were screened for their estrogen (E2) and progesterone (P4) content and expressing levels of integrin β1 and β3. Uterine receptivity was evaluated using human endometrial adenocarcinoma cells in an embryo attachment model. The respective and concatenated effects of embryo attachment and changes in the integrin β1 and β3 expression patterns on the adenocarcinoma cell plasma membranes in response to 100 nM concentrations of E2 and P4 were evaluated. Increased blood E2 concentrations were associated with significantly decreased the levels of integrin β3 expression in uterine biopsy samples. In vitro experiments revealed that a 100 nM E2 concentration inhibited the distribution of integrin β3 on the plasma membranes of human endometrial adenocarcinoma cells used in the embryo attachment model, and resulted in decreased rates of embryo attachment. In contrast, P4 enhanced the expression of integrin β1 and promoted its distribution on the plasma membranes. Furthermore, P4 recovered the embryo attachment efficiency that was lost by exposure to 100 nM E2. Blood E2 and P4 levels and integrin β3 and β1 expression levels in uterine biopsy samples should be considered as biomarkers for evaluating uterine receptivity and determining the optimal time for embryo transfer. Trial registration Trial number: ChiCTR-TRC-13003777; Name of registry: Chinese Clinical Trial Registry; Date of registration: 4 September 2013; Date of enrollment of the first study participant: 15 October 2013 The online version of this article (doi:10.1186/s12967-016-1052-0) contains supplementary material, which is available to authorized users.
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DOI：
10.1530/ror.0.0020084
发表时间：
1997-05-01
期刊：
Reviews of reproduction
影响因子：
--
作者：
Aplin, J D
通讯作者：
Aplin, J D
影响因子：
6.1
作者：
Lessey, BA
通讯作者：
Lessey, BA
影响因子：
3.4
作者：
de Ziegler, D;Fanchin, R;Bulletti, C
通讯作者：
Bulletti, C
DOI：
10.1023/b:jarg.0000027016.65749.ad
发表时间：
2004-03-01
影响因子：
3.1
作者：
Hendriks, DJ;Klinkert, ER;Broekmans, FJ
通讯作者：
Broekmans, FJ
影响因子：
4.8
作者：
Chen, DB;Bird, IM;Magness, RR
通讯作者：
Magness, RR