上皮细胞黏附和极性的破坏不仅促进恶性肿瘤的发生发展，并与原发性肿瘤的浸润和转移相关，其中Wnt/β-catenin信号通路扮演着重要作用。TSLC1是一种具有细胞黏附功能的新型抑癌基因，其在抑制肿瘤发生、转移以及上皮-间质转化等事件中的机制尚不清楚。为了阐明TSLC1在肝癌中的作用及内在机制，本研究拟通过建立肝细胞模型探讨TSLC1参与细胞黏附和维持细胞极性的作用以及与肝癌形成的关系；同时研究TSLC1抑制肿瘤上皮-间质转化的功能，并分析Wnt信号通路在TSLC1抑制肝癌中的作用。最后，我们设计靶向Wnt通路异常携带TSLC1基因的溶瘤腺病毒载体Ad-Wnt-TSLC1，并对其介导的细胞内表达TSLC1抗肝癌细胞增殖的作用机制进行分析和评价。

Destruction of the epithelial cell adhesion and cell polarity not only promotes the development of malignant tumors, and is involved with primary tumor invasion and metastasis, in which Wnt/β-catenin signal pathway plays an important role. TSLC1 is a novel tumor suppressor gene with cell adhesion function, whose mechanism in the inhibition of tumorigenesis, metastasis and epithelial-mesenchymal transition is unclear. To clarify the role and internal mechanism of TSLC1 in hepatocellular carcinoma, this study will investigate the function of TSLC1 involved in the maintenance of cell adhesion,cell polarity and liver cancer formation through the establishment of a liver cell model. Moreover, we also study the effect of TSLC1 in inhibition of tumor epithelial-mesenchymal transition, and analyze the role of Wnt signaling pathway in liver cancer inhibition by TSLC1. Finally, we design an oncolytic adenovirus vector Ad-Wnt-TSLC1 targeting the abnormal Wnt pathway, and analyze and evaluate the mechanism on antiproliferation of liver cancer cells by the intracellular expression of TSLC1.