基因转换是基因突变的重要组成部分，越来越多的研究表明，其在基因组的演化中扮演着重要的作用。但由于基因转换的片段较短，导致对其直接检测困难。本研究拟以存在多种基因型的水稻绿色革命基因sd1作为检测标记，构建能够直接用于检测基因转换的实验体系，并利用表型与基因型相结合的双重评测体系，从减数分裂和有丝分裂两个层面，系统评估水稻基因转换发生的频率及其生物学影响因素；该体系的最大优点在于野生型（高秆）和突变型表型（半矮秆）清晰可辨，能够大大提高检测效率，再通过基因型确证转换类型，保证结果的可靠性；其次，结合高通量测序技术，该体系可用于定量检测有丝分裂基因转换的频率，细胞竞争的方式与特征。本研究拟揭示植物基因转换的频率、规律及其影响因素，为系统评估基因转换在植物演化过程中的作用奠定基础。

Gene conversion (GC), by reshuffling existing variations into a novel genetic combination, plays an important role in genome evolution. However, comparing with crossover (CO) events, GCs are poorly documented owing to detecting difficulty with a very small converted tract size. The SD1 gene, a famous ‘green revolution’ gene in rice, with diversified alleles and correspondent varied heights, providing a unique opportunity for us to directly capture the rare NCO events. Here, through incorporating the sd1 locus as a reporter, we carefully designed a system, termed as the ‘sd1 reporter’ system, to estimate both the meiotic and mitotic GC rate in unprecedentedly higher resolution and greater efficiency. Such a system is robust against various artifacts owing to the clearly distinguished phenotypes and genotypes. Once a mitotic GC is identified, this system could be further extended to quantifying its frequency changes in cell lineages via the high-throughput sequencing technology. Therefore, we are not only allowed to study in details about the causes and consequences of GCs in plant genome evolution, but also could assesses its impact on the cell fate during plant development.