本研究在应用生物信息学技术分析Gly m Bd 28K蛋白抗原特性的基础上，应用基因工程技术、单克隆抗体技术和多肽合成技术全面分析和鉴定Gly m Bd 28K蛋白的IgG结合表位。主要研究内容包括（1）原核表达复性和纯化Gly m Bd 28K蛋白及大豆天然蛋白的提取；（2）抗Gly m Bd 28K特异性单克隆抗体的制备和鉴定；（3）全面覆盖Gly m Bd 28K蛋白重叠多肽的合成；（4）合成多肽与特异性抗体的反应性筛选和IgG结合表位鉴定。通过全面分析、鉴定Gly m Bd 28K蛋白的IgG结合抗原表位谱，探明大豆过敏产生的分子机制，揭示过敏反应的本质，对开展无过敏和低过敏的食品研究提供重要的理论指导。

Based on bioinformatic analysis of Soybean major allergen Gly m Bd 28K, IgG binding epitopes were analyzed and mapped with genetic engineering, monoclonal antibody technology and peptide synthesis. The main contents include (1) Prokaryotic expression、 refolding and purification of Gly m Bd 28K and Natural soybean protein extraction; (2) Preparation and identification of the monoclonal antibody specific to Gly m Bd 28K; (3) The synthesis of overlapping peptides of Gly mBd 28K; (4) The selection of synthetic peptides specific to monoclonal antibody and identification of IgG binding epitope. It will be provided important theoretical guidance to explore the molecular mechanism of soybean allergy, reveal the nature of an allergic reaction, develope no allergy and hypoallergenic foods research through comprehensive analysis, identification of the Gly m Bd 28K IgG binding epitope spectrum.