醛固酮瘤（APA）是原发性醛固酮增多症主要亚型之一。KCNJ5基因突变是APA发生机制的重要研究进展，而miRNA在APA发生过程中的研究至今较少报道。课题组前期在114例APA中对KCNJ5基因进行测序分析发现中国人群中KCNJ5突变有不同于国外的独特特点。基于以上发现，本课题组拟针对KCNJ5突变和非突变的APA与正常肾上腺组织进行miRNA对比分析（肿瘤组各10例，正常肾上腺组10例），根据文献报道和验证结果选择差异明显的miRNA，对该miRNA及其靶基因在肾上腺腺瘤H295R细胞增殖、凋亡、醛固酮分泌及相关基因的表达进行功能学研究；使用RT-PCR检测血标本中组织中差异表达的miRNA的相对表达量，比较血标本中突变与非突变两组中miRNA表达是否存在差异，寻找可能预测是否发生突变的APA，以更好的指导对APA患者术后的预后判断。

Aldosterone-producing adenoma (APA) is a major type of primary aldosteronism. KCNJ5 mutation has recently been suggested to be critical advances in the pathogenesis of APA. However, the role of miRNA in the development of APA was rarely reported. We previously discovered that the Chinese APA patients had unique KCNJ5 mutation characteristics by sequencing APA tissue from 114 Chinese patients. Based on the previous results, we next prepare to compare and analysis the differentially expressed miRNAs between KCNJ5 mutated APAs（10 cases）, non-mutated APAs（10 cases） and normal adrenal glands（10 cases）and to identify target miRNAs. Next we investigated the role of target miRNAs in the proliferation, apoptosis, aldosterone production and gene expression of H295R cells. Furthermore, we will use RT-PCR to detect the expression of target miRNAs in the preoperative blood samples to compared its expression between the KCNJ5 mutated and non-mutated APA patients, which may help predict the status of KCNJ5 mutation preoperatively and the postoperative prognosis.