非生物逆境是限制植物生产的重要因素。以旱寒区特有旱生资源植物柠条锦鸡儿为材料，研究高抗林木遗传资源及其新种质创制具有重大的理论意义和实践价值。本项目拟通过干旱胁迫诱导，在已建立的柠条锦鸡儿消减杂交文库(SSH)基础上，通过对获得的ESTs的研究，确定重要的抗逆候选基因。进一步通过拟南芥模式系统和农杆菌介导的柠条锦鸡儿子叶节转化系统，研究候选基因的抗逆功能，获得转基因柠条锦鸡儿材料，为植物抗逆基因工程和柠条锦鸡儿新种质创制提供理论依据和实践参考。

Abiotic stresses are extreme conditions which limit plant production. Caragana korshinskii Kom is an important cultivated shrub with a wide range of adaptability and high tolerance to drought, cold and saline. Using C. korshinskii as material to study high-resistant forest genetic resources and to create new germplasm has substantial theoretical and practical significance. In this study, we plan to further identify the important candidate stress-resistant genes based on the ESTs obtained from the suppression subtractive hybridization (SSH) library we constructed before under drought treatment. The selected candidate genes will be confirmed using model plant Arabidopsis, and then were transformed into C. korshinskii by established cotylendonary node regeneration system to get new stress-tolerant germplasm.