A家族的PP2C基因在ABA信号通路和植物胁迫响应中扮演着重要的角色，有关豆科植物中A家族PP2C基因在抗逆中作用机制的报道还很少。中间锦鸡儿是一种重要的豆科灌木，耐逆性强，挖掘抗逆基因的潜力巨大。本课题组对脱水处理的中间锦鸡儿进行了转录组测序，获得的大量抗逆相关基因中包括A家族的PP2C。在本研究中，我们将对豆科模式植物蒺藜苜蓿PP2C家族基因进行全基因组的分析和鉴定，进一步克隆蒺藜苜蓿和中间锦鸡儿中抗逆相关的A家族PP2C同源基因，构建在不同组织和不同胁迫下的表达谱。通过转基因拟南芥和蒺藜苜蓿，研究这些基因的生理功能，揭示A家族PP2C基因参与豆科植物抗逆及生长发育的分子机制，评价其应用价值和潜力，为蒺藜苜蓿和中间锦鸡儿A家族PP2C基因在豆科牧草和作物抗逆基因工程及分子育种中的应用奠定基础。

PP2C-A phosphatases (group-A PP2C) play important roles in abscisic acid (ABA) signaling and plant stress response. However, there are few reports on the mechanism of action of PP2C-A in legume plants..Caragana intermedia is an important forage bush belonging to the legume family. It has a great potential for research on stress-resistant genes owing to its strong tolerance to various stresses. In order to study its resistance mechanisms to drought and to screen drought responsive genes, a transcriptome of Caragana intermedia under drought stress was constructed in our previous work..In this study, a comprehensive analysis of genome-wide identification and characterization of the PP2C gene family will be carried in Medicago truncatula. Furthermore, we plan to clone the abiotic stress-induced PP2C-A genes from Medicago truncatula and Caragana intermedia, and analyze the mRNA expression profile of these PP2C-A genes under various abiotic stresses (such as drought, saline and cold), in different organs and at different development stages. Transgenic Arabidopsis thaliana or Medicago truncatula will be obtained to reveal their function and molecular mechanisms in resistance to abiotic stresses. This study will lay the foundation of applying PP2C-A gene from legume plant to genetic engineering of pasture and crop's stress resistance.