环境雌激素能通过体内的雌激素受体信号通路来干扰鱼类的生殖内分泌活动，最终损伤鱼类的生殖能力。然而，目前鱼类中不同的雌激素受体在生殖内分泌调控中的作用及分子机制并不清楚。鉴于此，本项目以转GnRH和GnIH启动子斑马鱼为材料研究外源雌激素对GnRH和GnIH表达的影响，其次，以雌激素受体单敲除斑马鱼为材料揭示三种雌激素受体（Esr2a、Esr2b和Esr1）在介导外源雌激素影响GnRH和GnIH表达中作用及分子机制。最后，以构建的雌激素受体单敲除、双敲除和全敲除斑马鱼为研究对象探讨不同的雌激素受体信号通路引起HPG轴活动变化并最终影响鱼类生殖的分子机理。本研究对雌激素受体在鱼类生殖内分泌的调控机制展开研究，探讨环境雌激素的生殖内分泌干扰途径，将为水产养殖的可持续发展提供最直接的理论依据。

Enviromental eatrogens are able to disrupt fish reproduction endocrine activity through estrogen receptor signaling and injury fish reproduction eventully. However, the roles of estrogen receptors signaling in regulating fish reproduction endocrine are unclear. The mechanism underlying is uncovered too. Therefore, we utilized transgenic zebrafish as an model to study the influence of xenoestrogen on GnRH and GnIH expression. Then, generated single estrogen receptor knock-out zebrafish(Esr2a, Esr2b and Esr1) were used for uncovering the roles of estrogen receptors in the process of xenoestrogen regulating GnRH and GnIH expression. At last, we will generate estrogen receptors double knock-out zebrafish and estrogen receptors all knock-out zebrafish. With these different kinds of knock-out zebrafish, we analyses the changes of HPG activity and influence fish reproduction eventually. The aim of this project is to study the regulatory mechanism of different estrogen receptors in reprocution endocrine. As a whole, our study will analyse pathway of xenoestrogen disrupting fish reproduction endocrine and provide direct theoretical basis for aquaculture sustainable develpoment.