Sexually dimorphic reproductive defects in zebrafish with spo11 mutation
Sexually dimorphic reproductive defects in zebrafish with spo11 mutation
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spo11突变斑马鱼的两性生殖缺陷
DOI：
10.1111/are.14829
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发表时间：
2020-08
影响因子：
2
通讯作者：
Yang Pinhong
中科院分区：
文献类型：
--
作者：
Zhang Yunsheng;Li Zhiping;Nie Yuan;Ou Guixiu;Chen Changzhi;Cai Shangye;Liu Liangguo;Yang Pinhong
减数分裂重组是减数分裂的关键步骤，因为它促进同源染色体配对并产生新的遗传等位基因组合，从而导致现存真核生物的多样化。Spo 11是一种减数分裂特异性蛋白，在多种生物中催化双链断裂（DSB）的形成并启动减数分裂重组，但Spo 11在鱼类配子形成中的确切功能需要进一步研究。本研究利用CRISPR/Cas9技术敲除了斑马鱼的pO 11基因。在这些突变的斑马鱼中发现了性二态性生殖缺陷。spo 11 <$/<$雄性完全不育，但spo 11 <$/<$雌性可生育，与WT雌性相比，相对生殖力和受精率无显著差异;然而，spo 11 <$/<$雌性的后代有严重的发育缺陷。另外，我们发现spo 11只在性腺组织中表达，并且在睾丸中的表达水平远高于卵巢。令人惊讶的是，spo 11也在斑马鱼胚胎中表达，特别是在圆顶阶段。我们比较了10种不同鱼类Spo 11的氨基酸序列，发现Spo 11是高度保守的。重要的是，这些结果将斑马鱼作为了解spo 11在减数分裂中的多种作用的脊椎动物模型。
Meiotic recombination is a key step in meiosis because it facilitates the pairing of homologous chromosomes and generates new combinations of genetic alleles, which results in the diversification of extant eukaryotes. Spo11, a meiosis‐specific protein, catalyses the formation of double‐strand breaks (DSBs) and initiates meiotic recombination in a wide variety of organisms, but the exact function ofspo11in the formation of gametes needs further study in fish. In this study, we knocked out thespo11gene in zebrafish using CRISPR/Cas9 technology. Sexually dimorphic reproductive defects were found in thespo11‐mutated zebrafish. The spo11‐/‐males were completely sterile, but spo11‐/‐females were fertile and there was no significant difference in relative fecundity and fertilisation rates compared with WT females; however, the offspring of spo11‐/‐females had severe developmental defects. In addition, we found thatspo11was expressed only in gonad tissue, and the level of expression in the testes was much higher than in the ovaries. Surprisingly,spo11was also expressed in zebrafish embryos, especially at the dome stage. We compared amino acid sequences of Spo11 in ten different fish and found that Spo11 was highly conserved. Importantly, the results established zebrafish as a vertebrate model for understanding multiple roles ofspo11in meiosis.
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影响因子：
3.8
作者：
N. Smirnova;P. Romanienko;P. Khil;R. Camerini‐Otero
通讯作者：
N. Smirnova;P. Romanienko;P. Khil;R. Camerini‐Otero
影响因子：
4.4
作者：
Romanienko, PJ;Camerini-Otero, RD
通讯作者：
Camerini-Otero, RD
影响因子：
16
作者：
W. Steiner;Randall W. Schreckhise;Gerald R. Smith
通讯作者：
W. Steiner;Randall W. Schreckhise;Gerald R. Smith
影响因子：
2.5
作者：
Kenji Saito;K. Siegfried;C. Nüsslein-Volhard;Noriyoshi Sakai
通讯作者：
Kenji Saito;K. Siegfried;C. Nüsslein-Volhard;Noriyoshi Sakai
影响因子：
2.5
作者：
Bowring, Frederick J.;Yeadon, P. Jane;Catcheside, David E. A.
通讯作者：
Catcheside, David E. A.