生命材料纳米低温保存是近年来新兴的热门研究方向，其理论和实验发展均不完善。纳米颗粒的引入对于细胞低温保存的诸多影响，目前尚缺乏系统的研究。此外，尚未见到完善的热力学模型，可用于描述纳米低温保存过程细胞外溶液非平衡相变、细胞内冰晶成核及其生长、跨膜物质输运、细胞内玻璃化等低温生物医学领域至关重要的物理过程。本项目拟选取人脐动脉内皮细胞为模型细胞(1)设计正交试验，系统研究典型纳米颗粒对其低温保存的影响，包括存活率、生长曲线、细胞凋亡、形态学等；(2)实验研究纳米低温保护剂悬浮液的热力学和动力学性质；(3)实验研究纳米颗粒对细胞膜生物物理学参数的影响；(4)建立可用于描述细胞纳米低温保存过程的热力学模型，定量评估细胞低温损伤，并与上述实验结果印证。本项目最终拟从基础机理出发，探寻纳米颗粒对于细胞低温保存的潜在应用价值，开拓低温保存新思路。项目开展对纳米低温保存学科的发展具有学术和应用价值。

Nanocryoproservation of biomaterials is an emerging popular research direction in recent years, while the development of the corresponding theories and experimental techniques is far from enough. No detailed investigation has been carried out on the effects of nanoparticles on cryopreservation.And no theoretically perfect thermodynamic models are available for describing the important physical processes during nanocryopreservation, including non-equilibrium phase change of extracellular solution, nucleation and the growth of intracellular ice crystals, mass transport across cell membrane, and intracellular vitrification, et al. This project is planning to investigate the influences of nanoparticles on the cryopreservation of human umbilical artery endothelial cells through orthogonal experiment design, and to experimentally determine the thermodynamic and kinetics properties of the nano-cryoprotectant solutions, and to investigate the effects of nanoparticles on the cryobiophysical parameters of the cell membrane. This project also proposes to establish a theoretical model for cell nanocryopreservation, which is available for quantificational evaluation of cell cryoinjury. The model predictions could be verified by the above mentioned cell nanocryopreservation experiments. Finally, this project intends to explore the potential application values of nanoparticles in cell cryopreservation from the basic principles, and to pioneer new thinking on cryopreservation. Successful carrying out of this project is of important academic significance and application value to the development of nanocryopreservation.