Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification.
Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification.
复制标题
复温期间超顺磁纳米颗粒的磁感应加热增强了玻璃化冷冻保存的 hUCM-MSC 的恢复
DOI：
10.1016/j.actbio.2016.01.026
复制
发表时间：
2016-03
影响因子：
9.7
通讯作者：
He X
中科院分区：
文献类型：
--
作者：
Wang J;Zhao G;Zhang Z;Xu X;He X
玻璃化冷冻保存已被认为是一种有前途的策略，长期银行的活细胞。然而，难以产生足够快的加热速率以最小化复温期间失透和重结晶诱导的细胞内冰形成是成功玻璃化的主要障碍之一。我们建议通过利用磁性纳米颗粒的磁感应加热（MIH）来克服这一障碍，以提高复温。采用化学共沉淀法制备了超顺磁性Fe 3 O 4纳米粒子。我们成功地将纳米Fe 3 O 4的MIH应用于玻璃化冷冻保存的人脐带基质间充质干细胞（hUCM-MSCs）的复温。我们的研究结果表明，细胞外Fe 3 O 4纳米粒子与MIH可以大大抑制失透和/或重结晶复温过程中，显着提高玻璃化细胞的存活。我们进一步优化了Fe 3 O 4纳米颗粒的浓度和用于产生MIH的交流（AC）磁场的电流，以使细胞活力最大化。我们的研究结果表明，MIH在AC磁场与0.05%（w/v）的Fe 3 O 4纳米粒子显着促进复温，并通过玻璃化提高冻存结果的hUCM-MSCs。应用MIH的SPM纳米粒子，以实现快速和空间均匀的加热是一个有前途的策略，增强冷冻保存干细胞的玻璃化。
Cryopreservation by vitrification has been recognized as a promising strategy for long-term banking of living cells. However, the difficulty to generate a fast enough heating rate to minimize devitrification and recrystallization-induced intracellular ice formation during rewarming is one of the major obstacles to successful vitrification. We propose to overcome this hurdle by utilizing magnetic induction heating (MIH) of magnetic nanoparticles to enhance rewarming. In this study, superparamagnetic (SPM) Fe3O4 nanoparticles were synthesized by a chemical coprecipitation method. We successfully applied the MIH of Fe3O4 nanoparticles for rewarming human umbilical cord matrix mesenchymal stem cells (hUCM-MSCs) cryopreserved by vitrification. Our results show that extracellular Fe3O4 nanoparticles with MIH may greatly suppress devitrification and/or recrystallization during rewarming and significantly improve the survival of vitrified cells. We further optimized the concentration of Fe3O4 nanoparticles and the current of an alternating current (AC) magnetic field for generating the MIH to maximize cell viability. Our results indicate that MIH in an AC magnetic field with 0.05% (w/v) Fe3O4 nanoparticles significantly facilitates rewarming and improves the cryopreservation outcome of hUCM-MSCs by vitrification. The application of MIH of SPM nanoparticles to achieve rapid and spatially homogeneous heating is a promising strategy for enhanced cryopreservation of stem cells by vitrification.
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DOI：
10.1126/science.1247391
发表时间：
2014-08-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fox IJ;Daley GQ;Goldman SA;Huard J;Kamp TJ;Trucco M
通讯作者：
Trucco M
DOI：
10.2174/1874120701105010047
发表时间：
2011
期刊：
The open biomedical engineering journal
影响因子：
--
作者：
He X
通讯作者：
He X
影响因子：
--
作者：
Etheridge, Michael L.;Xu, Yi;Bischof, John C.
通讯作者：
Bischof, John C.
影响因子：
2.7
作者：
Jin B;Kleinhans FW;Mazur P
通讯作者：
Mazur P
影响因子：
19
作者：
Huang H;Choi JK;Rao W;Zhao S;Agarwal P;Zhao G;He X
通讯作者：
He X