神经血管单元（NVU）是脑基本结构和功能单位，慢性脑缺血可导致NVU和神经细胞损伤，与认知功能障碍密切相关，但缺血性NVU损伤修复机制尚不清楚。我们研究发现，在氧糖剥夺细胞模型中，具有神经和血管双重调控作用因子VEGF/bFGF可促进脑微血管内皮细胞增生、成管和原代培养海马神经元NSE表达，并具有协同作用，提示神经与血管之间存在共同的调控靶点和内源性激活机制，协同参与神经和血管的再生修复过程。间接颅内外血管重建有助于改善缺血区脑循环，但存在血管再生不足和神经修复困难等问题。本项目基于NVU修复理念，选择具有缓释VEGF/bFGF作用的纳米纤维膜和血管重建联合干预，通过体内外实验，拟采用分子生物学、神经组织学、神经行为学、神经影像学和脑血流动力学等方法，解析联合应用VEGF/bFGF和血管重建对慢性缺血性认知功能障碍的作用及相关调控机制，为缺血性神经和血管再生修复及功能重建提供新的策略。

Neurovascular unit (NVU) represents the fundamental structural and functional unit of the brain. Chronic cerebral ischemia can result in the NVU and neural damage, which is closely related to cognitive impairment. However, the repair mechanisms of ischemic NVU injury remain unclear. Our previous study revealed that vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), the double regulating factors, could promote the proliferation of brain microvascular endothelial cells, tube formation, and neuronal specific enolasethe （NSE）expression of primary cultured hippocampal neurons with synergistic effects in the oxygen-glucose-deprivation cell model. These results indicate that there are common regulatory targets and endogenous activation mechanisms between neuron and vessel, which involve in the procedure of neuronal regeneration and repair. Indirect extracranial-intracranial revascularization helps to improve the cerebral circulation in ischemic areas, but there are some problems such as insufficient vascular regeneration and neuronal repair. Based on the concept of NVU repair, we initiate the combined application of nano-fiber membrane with sustain release of VEGF/bFGF and revascularization for interventing ischemia cognitive impairment using molecular biology, neurohistology, neurobehaviour, neuroimaging and cerebral hemodynamics in vitro and in vivo. We will decipher the effects and related regulation mechanisms of chronic ischemic cognitive impairment using combined application of VEGF/bFGF and revascularization, which will provide new strategies for ischemic neuronal and vascular regeneration and functional reconstruction in the future.