转录因子DAF-16（FoxO1同源基因）是调控线虫寿命的分子开关，它在激活条件下能结合DNA启动靶基因表达，使线虫寿命延长一倍。寻找DAF-16靶基因，研究它们如何协同作用是重要的科学问题。本课题拟（1）收集整理已得到实验证实的DAF-16靶基因，建立数据库，方便生物学家的研究工作。（2）多角度分析收集的靶基因特征，包括基因组、基因表达谱、蛋白质相互作用网络和信号通路等方面，并基于这些特征预测新的DAF-16靶基因。（3）结合DAF-16在激活和失活条件下的基因表达谱数据和蛋白质相互作用数据，分别构建线虫长寿和短寿的相互作用网络，通过比较这两个网络的差别，探索DAF-16靶基因如何相互协同延长线虫寿命。预期结果可促进以DAF-16为中心的衰老分子机制研究。

Transcription factor DAF-16 (FoxO1 homologous) plays as a molecular switch in the regulation of C. elegans lifespan, when activated, capable of binding the DNA to start the expression of its target genes to extend the lifespan of C. elegans. It is an important issue to investigate DAF-16 target genes and their cooperation in aging. In this proposal, we intend to (1) collect experimentally validated DAF-16 target genes and then set up a database to help biologist's research work; (2) analyze the features of the target genes at multi-levels, including genomics, gene expression profile, protein interaction network and molecular pathways, then predict new DAF-16 target genes based on the features obtained; (3) explore cooperative relationship among DAF-16 target genes by comparing the longevity gene network with short-lifespan gene network, which will be constructed by combining gene expression profiles of DAF-16 active or inactive with protein interaction network. The results are expected to promote understanding the molecular mechanism of aging regulated by DAF-16.