本课题将以“缺血再灌注（I/R）脊髓损伤后蛋白质不同时序变化特点为切入点”，基于以往研究的基础上拟采用转录组学分析方法，对其阶段I/R动态演进过程中涉及到的多基因、多通路的变化及其相互作用关系进行比较研究，希翼能够系统地揭示I/R脊髓发生发展的动态变化本质。本研究整合全基因组表达芯片和miRNA表达芯片等方法，对12-24h时序变化I/R脊髓损伤与正常配对样本进行差异基因分析，按照I/R演进的时间序列分析miRNA差异表达谱。综合采用生物信息学方法，分析转录水平的miRNA-mRNA的蛋白基因表达关系，研究结果将对于阐明I/R动态转录组学变化规律提供重要证据，具有重要的理论价值和现实意义。

This issue based on the previous studies and took Transcriptome change in different stages of spinal cord injury (I/R) ischemia-reperfusion as the starting point will adopt Transcriptome analysis method to study the dynamic evolution of multiple genes,multi-path changes and the interaction between a comparative study of the message in different stages I/R and hope to systematically reveal the dynamic changes in the nature of I/R spinal cord development.This study integrated whole-genome expression chips and miRNA expression chips and other methods to analysis different between I/R spinal cord injury and normal matching samples in 12 to 24 h temporal changes,and then to analysis differentially expressed spectrum of miRNA according to the time series of evolution of I/R.Comprehensive use bioinformatics method to analysis miRNA-mRNA gene expression regulation relationships at the level of transcription,the results will provide important evidence to clarify the dynamic I/R transcriptome variation with important theoretical and practical significance.