石灰介导下三七对镉胁迫的根部响应特征及机理研究

在土壤镉超标威胁到三七质量安全的背景下，以云南道地药材三七为研究对象，应用污染生态学和分子生物学理论，采用盆栽试验的方法，在不同Cd胁迫条件和石灰处理水平下，研究原产地文山三七二年生和三年生植株主根、支根、须根和剪口中Cd含量、生物量、总皂苷含量、单体皂苷R1、Rg1和Rb1含量、根系分泌物组成与含量、根系空间结构、三七鲨烯合酶的活性和基因的转录表达、根部蛋白质组差异表达；分析石灰介导下三七根部特征对土壤Cd胁迫的响应；探讨石灰介导下三七根系对土壤Cd响应的蛋白质组和分子调控机理。构建在酸性土壤镉污染条件下，保障三七的安全生产及三七产品品质的石灰调控的理论和技术。

Soil Cd contents in Panax notoginseng (Burk) F.H.Chen production areas exceeding the national standard value,which would influence in the security of medical plant P. notoginseng, were reported. P. notoginseng as geoherbs of Yunnan Province would be chosen as research materials. The simulation pot trials would be conducted with theory and methods of pollution ecology and molecular biology. The aims are: (1) to understand biomass, Cd contents, total saponins, singal saponins R1, Rg1 and Rb1, root exudates, root spatial structure, activities of squalene synthase (SS) and its transcription expression, differential expression of proteome in main roots, branching root, fibrous roots and rhizomes of two-year-old and three-year-old P. notoginseng with different soil Cd treatments and lime application; (2) to analysis the responses of roots of P. notoginseng to Cd stress with lime application; and (3) to clarify the protoeme and molecular mechanisms of responses of roots of P. notoginseng to Cd stress with lime application based on differential expression of proteome, activities of squalene synthase and its genes expression. That would be of important and be used for establishment theory and practice roles for guarantee yields and quality of P. notoginseng and promotion industrial development and ecological security of P. notoginseng with lime regulatory.