超富集植物小花南芥Cd、Pb迁移和分布的质膜系统CAX和HMA酶学及分子机理

土壤重金属污染的植物修复是目前关注的热点领域，以云南本土超富集植物小花南芥为研究对象，应用环境生态学和分子生物学理论和方法，采用盆栽试验和水培试验相结合的方法，在不同Cd、Pb胁迫条件下，研究小花南芥根部和叶片的生物量、Cd和Pb含量、Cd和Pb亚细胞分布含量、质膜系统（细胞质膜、液泡膜）CAX和HMA活性、CAX和HMA的转录组基因表达、HMA活性抑制剂对CAX活性及Cd、Pb含量的影响；研究CAX和HMA协同作用下，小花南芥Cd、Pb的亚细胞分布、迁移规律和累积特征，探讨小花南芥Cd、Pb迁移和分布的质膜系统CAX和HMA酶学和分子机理。构建在土壤Cd、Pb污染条件下，利用本土超富集植物修复土壤Cd、Pb污染的理论基础。

Based on phytoremediation of soil contaminated by heavy metals, native hyperaccumulator (Arabis alpinal Var. parviflora Franch) would be chosen as research materials. The simulation pot trials and hydroponic experiments would be conducted with theory and methods of environmental ecology and molecular biology. The aims are: (1) to understand biomass, contents of Cd and Pb, subcellular distribution of Cd and Pb, activities of CAX (cation/H+ antiporter, CAX) and HMA (heavy metal ATPase, HMA) of membrane systems (plasma membrane and tonoplast), differential expression of transcriptome and genes of CAX and HMA, CAX activities and Cd, Pb contents with active inhibitors of HMA treatments with different soil Cd and Pb concentration treatments; (2) to analysis subcellular distribution, translocation and accumulation characteristics of Cd and Pb of A. alpinal to Cd and Pb stressed; and (3) to clarify the enzymatic and molecular mechanisms of Cd and Pb distribution, translocation of A. alpinal to Cd and Pb stressed based on differential genes expression of transcriptome, activities of CAX and HMA and its genes expression. That would be of important and be used for establishment theory of native hyperaccumulator for phytoremediation of soil contaminated by Cd and Pb.