现有防龋蛋白疫苗诱导的粘膜免疫反应不理想，而防龋DNA疫苗因安全性的顾虑用于临床尚存诸多障碍。因此，研发高效且安全的防龋疫苗是急待解决的问题。本研究在已建立的科研平台基础上，经生物信息学分析筛选変链菌Pac和Glu有效的抗原表位，利用铁蛋白自组装功能，将変链菌的联合多肽表位结合到铁蛋白笼型结构外表面的N端暴露区，制备新型抗原性强且安全性好的自组装纳米粒子防龋疫苗。 检测该疫苗经粘膜免疫诱导特异性抗体水平、免疫类型与强度，评价防龋保护能力。分析其对粘膜CD103阳性树突状细胞的活化，继而对T、B 细胞的功能调控，以及对局部免疫微环境的影响，系统深入地探讨新型自组装纳米粒子防龋疫苗诱导的粘膜免疫反应的可能机制。本研究将为龋病的预防提供有效手段和奠定理论基础，也为其他经粘膜感染性疾病的粘膜疫苗的研发及粘膜免疫机制的研究提供新的策略和思路。

Current anti-caries protein vaccine for inducing mucosal immune response is not particularly ideal. Moreover, anti-caries DNA vaccine has some obstacles for clinical application. Therefore, it is essential to develop effective anti-caries vaccines.This study will screen the Pac epitopes and Glu epitopes of streptococcus mutans by bioinformatics which based on the established scientific research platform. Meanwhile, by using the self-assembling function of ferritin, the S.mutans epitopes will be linked to the the N exposed area of the cage shaped ferritin. Ultimately, the new type self-assembling anti-caries nanoparticle vaccine will be constructed. In vivo studies will be performed to detect the the levels of specific antibodies in serum and saliva,the type of the immune response as well as the ability of dental caries prevention through the mucosal immunity approach. In vitro study will analyse the activation ability for CD103 positive DCs, the regulation for T cells and B cells and the influence for the local immune microenvironment of the self-assembling anti-caries particle. This study will give rise to the new strategy and method for developing specific mucosal vaccines for other infectious diseases.