基因组关联分析结合重组自交系发掘番茄耐寒性QTL

摘要：番茄属非冷驯化植物，在其各生长发育阶段都易遭受低温伤害，番茄对低温适应的分子机制可能不同于冷驯化植物，如拟南芥。目前，对番茄低温应答途径和抗寒性控制位点及其效应知之甚少，本研究在建立的一套比较完整的番茄耐寒性表型鉴定体系的基础上，综合运用重测序的304份番茄核心种质和LA3969（耐寒）×LA4024（敏感）的重组自交系（RIL）群体及其杂交分离F2（F2:3）群体，通过GWAS和BSA等遗传研究方法，定位和发掘番茄耐寒的基因/QTL，并进行基因克隆及功能验证，利用RNA-Seq和酵母单/双杂交技术初步研究主效基因调控的分子机理，以期揭示番茄抵制寒冷的分子机制及应答调控网络，开发番茄抗寒基因分子标记，为番茄耐寒性的分子标记辅助育种提供基础。

The cultivated tomato (Solanum lycopersicum) is unable to cold acclimation, and easily suffers cold injury at all stages of plant growth and development. The molecular basis of cold adaptation in non-cold-acclimated tomato may differ from that of the cold-acclimated plants such as Arabidopsis. Until nowadays, the mechanism of cold tolerance and the main QTL loci in tomato are largely unknown. The project plans to map the loci resistant to cold stress and try to clone the major effect loci/genes, by utilizing of 304 re-sequencing core germplasms, and recombinant inbred line (RIL) population and the segregation population of cold-tolerant (LA1777 and LA3969) and -sensitive (LA4024) tomato with the aid of genome-wide association analysis (GWAS) and bulked segregant analysis (BSA). Eventually, we help to clone and characterize the function of candidate genes by the transgenic verification. Furthermore, we can study the cold tolerant molecular mechanisms by RNA-Seq and yeast one/two hybrid technology. This research has a great significance in revealing the molecular mechanism resistant to cold in tomato, developing cold-tolerant molecular markers and the cold-tolerant cultivars