An ATL78-Like RING-H2 Finger Protein Confers Abiotic Stress Tolerance through Interacting with RAV2 and CSN5B in Tomato.
An ATL78-Like RING-H2 Finger Protein Confers Abiotic Stress Tolerance through Interacting with RAV2 and CSN5B in Tomato.
复制标题
番茄中类似 ATL78 的 RING-H2 指蛋白通过与 RAV2 和 CSN5B 相互作用赋予非生物胁迫耐受性
DOI：
10.3389/fpls.2016.01305
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发表时间：
2016
影响因子：
5.6
通讯作者：
Ye Z
中科院分区：
文献类型：
--
作者：
Song J;Xing Y;Munir S;Yu C;Song L;Li H;Wang T;Ye Z
环指蛋白在植物对非生物胁迫的适应中起着重要的作用。本研究克隆了一个野生番茄（Solanum habrochaites）冷诱导RING-H2指基因ShATL 78 L，该基因已被鉴定为番茄非生物胁迫响应基因。结果表明，ShATL 78 L在根、叶、叶柄、茎、花和果实等组织中均呈组成型表达。低温胁迫上调了耐冷S. habrochaites相比，敏感的栽培番茄（S。lycopersicum）。此外，ShATL 78 L的表达还受到干旱、盐、热、创伤、渗透胁迫和外源激素等不同胁迫的调控。功能表征表明，栽培番茄过表达ShATL 78 L具有提高的耐受性，寒冷，干旱和氧化胁迫相比，野生型和敲低线。为了了解ShATL 78 L调控非生物胁迫反应的分子机制，我们进行了酵母单杂交和双杂交试验，发现RAV 2可以结合ShATL 78 L的启动子并激活/改变其转录，CSN 5 B可以与ShATL 78 L相互作用来调控非生物胁迫反应。综上所述，这些结果表明ShATL 78 L通过与RAV 2结合并与CSN 5 B相互作用在调节植物对非生物胁迫的适应中起重要作用。亮点：RAV 2与ShATL 78 L的启动子结合，激活/改变其转录以适应环境条件; ShATL 78 L还与CSN 5 B相互作用以调节胁迫耐受性。
RING finger proteins play an important role in plant adaptation to abiotic stresses. In the present study, a wild tomato (Solanum habrochaites) cold-induced RING-H2 finger gene, ShATL78L, was isolated, which has been identified as an abiotic stress responsive gene in tomato. The results showed that ShATL78L was constitutively expressed in various tissues such as root, leaf, petiole, stem, flower, and fruit. Cold stress up-regulated ShATL78L in the cold-tolerant S. habrochaites compared to the susceptible cultivated tomato (S. lycopersicum). Furthermore, ShATL78L expression was also regulated under different stresses such as drought, salt, heat, wound, osmotic stress, and exogenous hormones. Functional characterization showed that cultivated tomato overexpressing ShATL78L had improved tolerance to cold, drought and oxidative stresses compared to the wild-type and the knockdown lines. To understand the underlying molecular mechanism of ShATL78L regulating abiotic stress responses, we performed yeast one-hybrid and two-hybrid assays and found that RAV2 could bind to the promoter of ShATL78L and activates/alters its transcription, and CSN5B could interact with ShATL78L to regulate abiotic stress responses. Taken together, these results show that ShATL78L plays an important role in regulating plant adaptation to abiotic stresses through bound by RAV2 and interacting with CSN5B. Highlight: RAV2 binds to the promoter of ShATL78L to activates/alters its transcription to adapt the environmental conditions; furthermore, ShATL78L interacts with CSN5B to regulate the stress tolerance.
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