组蛋白去乙酰化酶（HDAC）介导的组蛋白修饰是基因表达表观遗传调控的重要方式。我们近期的研究结果表明，拟南芥两种不同类型的组蛋白去乙酰化酶HD2C（HD2型）和HDA6（RPD3型）相互作用共同参与植物ABA及盐逆境胁迫的响应，然而对于其表观遗传分子机理并不清楚，本项目主要进行以下研究：（1）研究HD2C和HDA6参与调控植物逆境胁迫应答的靶基因及基因调控网络；（2）研究HD2C和HDA6相互作用的蛋白及蛋白质复合体组成成分；（3）鉴定HD2C和HDA6在逆境胁迫应答中对组蛋白修饰的影响及组蛋白修饰相互之间的关系。通过上述研究，阐明两种不同类型的组蛋白去乙酰化酶相互作用在植物逆境胁迫应答中参与基因表达调控的分子机制，揭示植物适应环境的表观遗传机制，并有助于为植物的抗逆育种提供新的思路。

Histone modifications such as histone deactylation play an important role in the epigenetic regulation of gene expression. Our recent studies found that HD2-type histone deacetylase HD2C and RPD3-type histone deacetylase HDA6 were involved in ABA and salt response. Interestingly, we also found HD2C can interact with HDA6 in vitro and in vivo. However, the molecular mechanisms are not clear. To better understand these mechanisms, in this proposal we will analyze the gene expression network and identify the direct target genes of HD2C and HDA6, identify the interaction proteins and protein complex of HD2C and HDA6, analyze the changes of histone modification which affected by HD2C and HDA6 under ABA and salt treatment. These studies will enhance our understanding of how HD2C and HDA6 modulate plant gene expression in response to abiotic stresses, which may ultimately be applicable in improvement of agricultural productivity.