枣树主要栽培区土壤次生盐碱化严重，丛枝菌根（AM）可显著提高枣树的耐盐性，但盐胁迫下AM维持枣树Na+、K+稳态的机制尚不明确。本研究拟以枣树砧木-酸枣作为试材，采用盆栽试验，接种菌根菌（Rhizophagus irregularis）和添加NaCl，监测AM的Na+、K+等离子流，从动态的角度研究AM对盐离子选择吸收的调控；基于转录组水平，分析菌根共生对酸枣Na+、K+通道/转运蛋白基因表达的调控方式；采用激光显微切割技术结合qRT-PCR，研究AM含丛枝细胞中酸枣-菌根菌的离子通道/转运蛋白等基因对盐胁迫的响应方式；进一步采用酵母功能互补试验验证菌根菌 Na+/K+离子转运/通道蛋白的功能。本研究旨在从酸枣-菌根菌双方Na+、K+转运蛋白的协同表达方式来阐释菌根维持酸枣Na+、K+稳态平衡的机制，可丰富植物抗盐胁迫的理论，对保障盐碱地枣树可持续发展具有重要科学意义。

Chinese jujube (Ziziphus jujuba) is an important fruit tree, and mostly growing in the saline-alkaline soil conditions. Previous studies have revealed arbuscular mycorrhizal symbiosis has been shown to confer abiotic stress tolerance to sour jujube (Z. jujuba var. spinosa). However, the physiological and molecular mechanism of improving jujube Na+、K+ homeostasis by mycorrhizal symbiosis is still unknown. In this study, we perform a pot experiment to study the salt tolerance of sour jujube seedlings inoculated with mycorrhizal fungi (Rhizophagus irregularis) by addition of NaCl into the medium, and then investigate the physiological response of photosynthetic parameters, and Na+、K+ distribution. To determine the effect of mycorrhizal colonization on K+/Na+ homeostasis of sour jujube seedlings, we analyze salt-induced fluxes of Na+, K+, etc. of root tips using a scanning ion-selective electrode technique. In addition, the total RNA from fine roots under different treatments were extracted and then submitted to RNA-seq on Hiseq platform. We performed the comparative transcriptomic analysis among different treatments to revealed the differential gene expression profiles of sour jujube roots induced by mycorrhizal colonization under salt stress. We annotate and identify Na+/K+ transporters/channel proteins in Z. jujuba and R. irregularis, respectively. We then determine those members in mycorrhizal roots of sour jujube regulated by salt stress. Thereafter, the cell tissues harboring arbuscules were captured in the mycorrhizal tips using laser capture microdissection technology and then subjected to RNA extraction. We further analyze the gene expression pattern of Na+/K+ transporters/channel proteins belonging to sour jujube and arbuscular mycorrhizal fungi by qRT-PCR, respectively, and characterize their relations with the Na+, K+ homeostasis. Finally, we perform complementation assays on the Na+/ K+ channels and transporters using yeast mutant strainW6 defective in K+ uptake. In general, we try to characterize the molecular mechanism of mycorrhizal symbiosis improving jujube Na+、K+ homeostasis through analyzing the gene expression of Na+、K+ channels and transporters of both sour jujube and R. irregularis. This research would provide new insights into the mechanism of plant salt tolerance, and finally contributes to the jujube industry in the saline-alkaline area.