木霉次级代谢产物Trichokonins生物合成基因簇的鉴定及Lae1因子对其生物合成的调控作用

木霉产生的肽类次级代谢产物peptaibols富含α-氨基异丁酸，具有独特的理化性质和多样的生物学功能。但有关其生物合成及调控的研究很少。长枝木霉产生的peptaibols -Trichokonins具有抗菌、抗病毒、抗肿瘤等生物学活性，有很好的应用潜力。基因组分析发现该菌株含有多个NRPS基因和全局性调控因子Lae1基因。在前期研究基础上，本项目拟鉴定未知的Trichokonins，根据基因组信息，对Trichokonins生物合成基因簇和全局性调控因子Lae1进行深入的研究。通过构建不同的缺失突变株或过表达菌株，结合转录组分析，以解析木霉SMF2菌株Trichokonins生物合成基因簇及全局性转录因子Lae1对Trichokonins生物合成的调控。本研究有助于深入理解木霉次级代谢产物的生物合成机制，增强对丝状真菌次级代谢产物调控的认知，为更好地利用木霉次级代谢产物奠定理论基础。

Trichoderma spp. produce special peptide secondary metabolites-peptaibols,which are rich in α-amino isobutyric acid and have unique physicochemical properties and various biological functions. But the mechanism of biosynthesis and regulation on peptaibols remains poorly understood. Trichokonins, peptaibols from Trichoderma longibrachiatum SMF2, exhibited various biological activities, such as antibacterial, antiviral, antitumor, and induce resistance in plant, implying the potential in agricultural and medical production. It was found that there are several NRPS genes and a global regulation factor Lae1gene in the genome of Trichoderma SMF2. In this study, we will identify the new Trichokonins and study the biosynthesis gene clusters of Trichokonins and the role of global regulation factor Lae1 on Trichokonin biosynthesis by combining the construction of different mutants and transcriptome analysis. It is helpful to understand the biosynthesis mechanism of secondary metabolites from Trichoderma, increase awareness of filamentous fungal secondary metabolites of cognition, and lay the theoretical foundation for better use of secondary metabolites from Trichoderma.