木霉驱动的根际微生物群落变化及其生态机制的宏组学研究

木霉是重要的生防和促生长因子，成功地用于农业生产，但是田间应用稳定性不好的原因严重地影响木霉的推广应用。土壤的生物、非生物因素都会影响木霉的田间使用效果，目前对于木霉施入土壤后的生态学功能尚缺乏深入的认识。现代组学技术的发展，为从整体水平上认识土壤微生物组及其功能提供了强有力的技术支撑。在前期工作中，我们深入研究了木霉及其次级代谢产物在木霉-植物病原微生物，木霉-植物相互作用中的作用及机制。本研究拟在前期工作的基础上，采用非培养的宏基因组和宏转录组技术，结合可培养的微生物区系分析及微生物生物过程分析，研究木霉处理对番茄根际微生物群落组成和多样性的影响，探寻差异富集微生物，挖掘差异表达功能基因。结合土壤理化性质分析和植物生长指标测定，解析木霉驱动的根际微生物群落结构和功能的变化及其与土壤理化因子、植物生长指标的关系，最终从系统生物学角度阐明木霉的生态机制，为更好应用木霉资源奠定基础。

Trichoderma spp are important biological control agents and plant growth promoting agents, having been successfully used in agricultural production. However, the field application effects are not stable, which hindering the widely use of Trichoderma in agricultural production. The biotic and abiotic factors both affect the stability of Trichoderma in soil. Till now, the ecology function of Trichoderma is still unknown. Modern omics techniques provide strong technical support for understanding the composition, diversity and function of soil microbiome. In previous study, we have studied the roles of Trichoderma and their secondary metabolites in Trichoderma-phytopathogens and Trichoderma-plant interactions. In this study, we will use metagenomics and metatranscriptomics techniques, combing cultural microbial community and biological process analysis methods, to study the variations of microbial microbiota composition, diversity and function driven by Trichoderma in tomato rhizosphere. The significant differentially enriched microbial and expressed function genes will be mined and their functions will be analyzed. Combined these unculturable and culturable microbial analysis results, the soil physicochemical properties analysis and plant growth assay results, the ecological mechanism of Trichoderma will be elucidated, laying a foundation for the better application of Trichoderma resources in agricultural production.