鲫疱疹病毒CaHV已经严重制约了异育银鲫的可持续养殖。在分析CaHV易感克隆系A+、中等抗性克隆系F以及高抗克隆系H在感染CaHV前后头肾转录组基础上，本项目拟从银鲫F系全基因组序列中鉴定主要IFN系统基因的所有基因拷贝，通过比较基因组学揭示多倍体银鲫IFN系统基因的多样性及其在进化上对银鲫适应性的作用；分析抑制病毒复制的viperin等主要IFN系统基因的不同基因拷贝在3个克隆系感染CaHV后免疫器官中的表达变化，筛选与抗CaHV紧密相关的关键基因，揭示抗病毒功能和信号通路；通过启动子分析筛选与银鲫CaHV抗性相关的关键位点，揭示银鲫不同克隆系间IFN系统调控机制差异；最后拟通过BAC转基因技术和基因组编辑技术探索培育银鲫抗病新品种的技术途径。本项目的研究结果不仅可以为揭示鱼类基因组加倍后重复基因的适应性进化机制提供一个典型范例，也可为异育银鲫分子设计育种奠定技术平台。

Gibel carp is an important aquaculture species in China, and Carassius auratus herpesvirus (CaHV) has hampered aquaculture development. In previous study, we performed herpesvirus challenge experiments in three gynogenetic clones of gibel carp, including leading variety clone A+, candidate variety clone F and wild clone H, and found that the three clones showed distinct resistance to herpesvirus. Based on the comparative transcriptome profiling between diseased individuals and control individuals in clone A+, F and H, we will firstly identify all gene copies of important interferon system genes from the draft genome of gibel carp clone F and reveal the roles of their diversification to adaptive evolution of gibel carp through comparative genomic analysis. Then, we will identify the important IFN genes related to CaHV resistance difference by analyzing the differential expression changes of IFN system genes among the three gynogenetic clones of gibel carp during different CaHV infected stages, and reveal their function and signal pathway. Furthermore, the differential regulative mechanism to CaHV resistance among three gynogenetic clones of gibel carp will be revealed through promotor analysis. Finally, we will try to apply the techniques of BAC transfer and genome editing to breed novel strain of gibel carp with higher resistance to CaHV. This study will provide a typical example for adaptive evolution of duplicated genes of teleost polyploid, and will benefit to the gibel carp disease-resistance molecular breeding.