针对现阶段国内果蔬产量增速趋缓，越来越多的人关注于品质追求的现象，开展果蔬成熟与品质形成的调控机制研究具有重要的理论和实际意义。课题组前期研究表明RNA结合蛋白参与了调控番茄果实成熟，并且在全基因组水平筛选到与番茄成熟衰老相关富含甘氨酸的RNA结合蛋白-SlGRP7。本项目拟对SlGRP7开展深入研究，对SlGRP7进行表达模式及亚细胞定位分析，沉默与超表达SlGRP7基因从而研究它的生物学功能和对果实成熟的调控；并在全基因组范围内通过RNA免疫共沉淀（RIP-seq）技术筛选SlGRP7直接结合的靶RNA，研究SlGRP7对果实成熟相关RNA的调控方式；利用标签蛋白抗体和免疫沉淀结合质谱分析(IP-MS)技术获得SlGRP7蛋白复合物，全基因组范围内鉴定SlGRP7的互作蛋白；最终诠释SlGRP7调控番茄果实成熟衰老的分子机制，为果实品质保持和耐贮性改良提供理论基础。

In view of the slowdown in the growth of domestic fruit and vegetable production at this stage, an increasing number of people are paying attention to the phenomenon of quality pursuit. It is of great theoretical and practical significance to carry out research on the regulation mechanism of fruit and vegetable maturity and quality formation. Previous studies in the research group showed that RNA-binding proteins are involved in the regulation of tomato fruit ripening, and the glycine-rich RNA-binding protein-SlGRP7 associated with tomato ripening was screened at the genome-wide level. This project intends to conduct an in-depth study of SlGRP7. The biological function and regulation of fruit ripening will be studied by analyzing the expression pattern and subcellular localization of SlGRP7 through silencing and overexpressing the SlGRP7 gene. The regulation of SlGRP7 on fruit ripening-related RNA will be studied using RNA immunoprecipitation (RNA-seq) technique to screen the target RNA directly bound by SlGRP7 in the whole genome. The interaction protein of SlGRP7 will be identified in the whole genome by the SlGRP7 protein complex, which will be obtained by using tagged protein antibody and immunoprecipitation combined with mass spectrometry (IP-MS). Finally, the molecular mechanism of SlGRP7 regulating the ripening and senescence of tomato fruit will be explained, providing a theoretical basis for fruit quality maintenance and storability improvement.