Bt棉在我国大规模应用后，绿盲蝽种群数量迅速上升，演化为Bt棉田主要害虫。虽然目前已经从生态学角度研究了绿盲蝽对Bt棉的适应性，但国内外很少有关于绿盲蝽取食Bt棉后分子消化机制方面的报道。本申请拟在前期明确了类胰蛋白酶AlSP4是绿盲蝽取食Bt棉后重要消化酶的研究基础上，通过原位杂交、免疫组化、原核表达、酶活测定以及RNAi干扰等技术，重点研究AlSP4基因的时空表达、生理功能及AlSP4蛋白的生化特征，进而阐明AlSP4蛋白在绿盲蝽适应取食Bt棉过程中的作用。以上结果可以阐释AlSP4通过影响寄主营养物质的吸收，进而影响绿盲蝽总蛋白含量、成虫寿命及生殖产卵等机制，最终影响其在Bt棉田的适应性的假设。研究结果是对生态学角度研究盲蝽蟓适应Bt棉田取食危害机理在分子角度的一个重要补充，不仅可以明确消化酶介导绿盲蝽取食Bt棉后的分子消化机制，还可为Bt棉田绿盲蝽防控探索新的靶标提供科学依据。

Apolygus lucorum, which adapt to feeding on Bt cottons as soon as Bt cottons are large-scale applicated in China, become the primary pest on Bt cottons in the fields. The adaptability of A. lucorum to Bt cottons has been studied via ecology methods, but physiological mechanism of the adaption to Bt cottons by A. lucorum is limited. On the basis of our previous studies that AlSP4 is an important trypsin-like protease gene for A. lucorum adapting to Bt cottons, this project is aimed to measure the spatial and temporal expression of AlSP4, biochemical characteristics of AlSP4, physiological functions of AlSP4, and to clarify the effect of AlSP4 protein after A. lucorum feeding on Bt cotton by a series of molecular experiments, such as in situ hybridization, immunohistochemical, prokaryotic expression, enzyme activity analysis and RNA interference techniques. These results will confirm the hypothesis that trypsin-like protaese AlSP4 could play an important role in the adaptability of A. lucorum survived on Bt cottons in the fields by regulating the absorption of nutrition from the host materials. And then, the measurement of the total protein content, adult longevity and fecundity index in A. lucorum could be verified after A. lucorum feeding on Bt cottons. These results are also very important supplements of feeding damage mechanism on Bt cottons by A. lucorum from ecology viewpoint. This research not only help us to realize the adaptation mechanism of feeding on Bt cottons of A. lucorum mediated by digestive enzyme, but also provide scientific basis to explore the new targets of management of A. lucorum on Bt cotton fields.