重离子束作为一种新兴的辐射诱变源，具有生物诱变效应强、突变谱广、突变率高、DNA损伤修复率低等优点。重离子辐照已经成为植物品种改良的重要手段之一，此外，诱变的突变体也可用于基因功能的研究，成为反向遗传学的重要研究手段。南荻是我国特有的具有巨大发展潜力的纤维类芒属能源植物，土壤盐碱化是目前制约南荻在边际土地种植的重要因素之一。本研究拟以南荻为研究对象，选用不同组织（种子、愈伤组织、茎侧生芽和地下茎节芽）和不同的辐射剂量进行重离子辐照，筛选耐盐碱的突变品系，并借助微阵列比较基因组学杂交（array-CGH）和高分辨率熔解曲线（HRM）分析等手段，在基因组水平上对重离子辐射诱变引起的染色体结构变异（SNPs, InDel, CNV等）进行鉴定，对重离子辐射造成的DNA损伤方式及其诱变效应进行深入分析，将进一步丰富对重离子辐射诱变效应及其作用机理的认识，为南荻重离子辐射诱变育种和功能基因组学研究奠定基础。

Heavy-ion beams are accepted as a novel and powerful mutagen because of their superior characteristics, such as relatively higher biological effectiveness, wide spectrum of mutation, higher mutation efficiency, and the lower recovery rate of DNA damages induced. Recently, heavy-ion beams have been utilized as a novel and efficient mutagen in plant breeding. In addition, the loss-of-function mutants generated by heavy-ion beam irradiation enormously faciliate plant functional genomics studies using reverse genetics means. Miscanthus lutarioriparius is a perennial rhizomatous grass endemic to China. It has been deemed as one of the most potential candidates of lignocellulosic crops in new-generation bioenergy production. Currently, soil salinity has been one of the main limiting factors for large-scale cultivation of Miscanthus lutarioriparius in marginal land in China. The present study was undertaken to generate salt-tolerant varieties of Miscanthus lutarioriparius by heavy-ion beam irradiation. The mutation effectiveness of various tissues (seed, callus induced from immature spikelet, lateral bud and rhizome bud) with different irradiation doses will be examined. In addition, we will characterize the aberrance of chromosome structures including SNPs, InDels and CNVs induced by heavy-ion irradiation at the genome level. To fulfill this goal, array-based comparative genomic hybridization (array-CGH) and high-efficiency melting curve analyses will be performed. The results obtained will provide novel insight into the molecular mechanism and biological effectiveness induced by heavy-ion irradiation, thus may lay the foundation for the breeding of new cultivars by heavy-ion irradiation as well as functional genomics studies in Miscanthus lutarioriparius.