Transcriptome analysis of genes involved in secondary cell wall biosynthesis in developing internodes of Miscanthus lutarioriparius.
Transcriptome analysis of genes involved in secondary cell wall biosynthesis in developing internodes of Miscanthus lutarioriparius.
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芒草节间发育中次生细胞壁生物合成相关基因的转录组分析
DOI：
10.1038/s41598-017-08690-8
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发表时间：
2017-08-22
影响因子：
4.6
通讯作者：
Zhou G
中科院分区：
文献类型：
--
作者：
Hu R;Xu Y;Yu C;He K;Tang Q;Jia C;He G;Wang X;Kong Y;Zhou G
芒属植物是一种很有前途的木质纤维素生物能源作物。为了鉴定芒属植物次生细胞壁（SCW）发育的候选基因和调控网络，我们对一个发育中的节间进行了从头转录组分析。根据组织学和原位组织化学分析，M. lutarioriparius可分为三个不同的节段，即上部节间（UI）、中部节间（MI）和基部节间（BI），每个节段代表SCW发育的不同阶段。转录组分析产生了大约3亿个干净的读段，这些读段被重新组装成79，705个单基因。近65%的unigenes在7个公共数据库中被注释。UI、MI和BI之间的比较分析揭示了四个不同的集群。此外，详细的表达谱分析的基因家族和转录因子（TF）参与SCW的生物合成，组装和修饰。基于共表达模式，构建了转录因子和SCW相关基因之间的调控网络。本研究首次对M. lutarioriparius。这些结果为芒属植物SCW的生物合成和调控提供了新的见解。此外，所确定的基因代表了进一步功能研究的良好候选者，以阐明它们在SCW生物合成和修饰中的作用。
Miscanthus is a promising lignocellulosic bioenergy crop for bioethanol production. To identify candidate genes and regulation networks involved in secondary cell wall (SCW) development in Miscanthus, we performed de novo transcriptome analysis of a developing internode. According to the histological and in-situ histochemical analysis, an elongating internode of M. lutarioriparius can be divided into three distinct segments, the upper internode (UI), middle internode (MI) and basal internode (BI), each representing a different stage of SCW development. The transcriptome analysis generated approximately 300 million clean reads, which were de novo assembled into 79,705 unigenes. Nearly 65% of unigenes was annotated in seven public databases. Comparative profiling among the UI, MI and BI revealed four distinct clusters. Moreover, detailed expression profiling was analyzed for gene families and transcription factors (TFs) involved in SCW biosynthesis, assembly and modification. Based on the co-expression patterns, putative regulatory networks between TFs and SCW-associated genes were constructed. The work provided the first transcriptome analysis of SCW development in M. lutarioriparius. The results obtained provide novel insights into the biosynthesis and regulation of SCW in Miscanthus. In addition, the genes identified represent good candidates for further functional studies to unravel their roles in SCW biosynthesis and modification.
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影响因子：
4.2
作者：
da Costa RM;Lee SJ;Allison GG;Hazen SP;Winters A;Bosch M
通讯作者：
Bosch M
影响因子：
9.5
作者：
Cosgrove DJ
通讯作者：
Cosgrove DJ
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
6.9
作者：
Bosch M;Mayer CD;Cookson A;Donnison IS
通讯作者：
Donnison IS
影响因子：
7.4
作者：
Baima, S;Possenti, M;Morelli, G
通讯作者：
Morelli, G