苦豆子总碱是从宁夏回族习用药苦豆子提取的总生物碱，本室前期研究结果表明苦豆子总碱可显著降低胶原诱导关节炎（CIA）大鼠脾滤泡辅助性T（Tfh）细胞数量，减轻其关节肿胀，抑制滑膜组织TLRs的表达。但苦豆子总碱能否经TLRs调节Tfh细胞的分化及功能从而影响生发中心（GC）的形成及自身抗体的产生尚不清楚。本研究以初发类风湿性关节炎（RA）患者及CIA小鼠为研究对象，用受体或信号蛋白抑制剂、MACS、FCM、ELISA、Western-Blot及RT-PCR等技术，证实苦豆子总碱抗RA的效果；明确Tfh细胞在RA发病中的作用；揭示苦豆子总碱经Tfh细胞的“TLRs-NF-κB-Bcl-6轴”调控Tfh-autoreactive B细胞而发挥抗类风湿性关节炎的免疫学机理。以期阐明其作用靶点和分子机制，为苦豆子总碱应用于类风湿性关节炎的临床免疫治疗，为回药的深度开发利用提供基础供理论和实验依据。

Total Alkaloids from Sophora Alopecuroides（TASA）is the total alkaloid extracted medicine from Ningxia Hui Nationality plant Sophora alopecuroides L..The preliminary findings of our research group showed that TASA has a apparent anti-inflammatory and analgesic effect,which reduced the number of Tfh in rat spleen,alleviated significantly joint swelling and the expression of TLRs in the synovial tissue of CIA rat. But whether TLR regulate differentiation and function of Tfh by TASA is unclear.In this study,patients with newly diagnosed,the collagen-induced arthritis(CIA) model, the proposed application of signal inhibitors or receptor inhibitors, MACS technology, flow cytometry, Western-Blot, RT-PCR, ELISA technology and tomography, clear the pathogenesis of Tfh in rheumatoid arthritis, explore immunological mechanisms of anti-rheumatoid arthritis that TASA regulates the interaction between Tfh and autoreactive B cells via “TLR9-NF-κB-Bcl-6 axis” of Tfh. Through these research, our group want to clarify the mechanism of TASA and application in the clinical immunology treatment, provide the basis of theoretical and experimental basis for the deep development and utilization of Ningxia Hui Nationality medicine.