杜仲单用或与西药合用在治疗高血压方面疗效显著，为贵州省重点开发的中药材之一，然而杜仲具体的降压物质尚不清楚，已成为其深层次开发利用的技术瓶颈。药代动力学-药效动力学（PK-PD）结合模型可以为中药的药效物质基础阐明提供科学依据。鉴于中药主要是作用于病理状态的机体，因此建立病理状态下的杜仲降血压活性成分的PK-PD结合模型至关重要。申请人在前期研究中发现杜仲的潜在降压成分松脂醇二葡萄糖苷和京尼平苷酸在自发性高血压大鼠（SHR）体内的暴露量显著高于正常大鼠。有鉴于此，本课题拟在前期研究基础上，以SHR为动物模型，同时以其主动脉血管内皮细胞（VEC）为细胞模型，并对VEC施加一定的剪切力，以模拟高血压状态下的受损细胞，从病理状态和细胞药代动力学的角度，构建杜仲降血压活性成分的PK-PD结合模型，以期阐明杜仲在体内与降血压作用直接相关的药效物质，为其药用资源的深度开发奠定科学依据。

Eucommia ulmoides alone or in combination with Western medicine has a significant effect in the treatment of hypertension, which is one of important and famous traditional Chinese medicine in Guizhou Province. However, there are some difficulties to the further develop because the specific antihypertensive substance of Eucommia ulmoides is not clear. Pharmacokinetic-pharmacodynamic (PK-PD) link model can provide a scientific basis for elucidating the pharmacological basis of traditional Chinese medicine. Given the fact that traditional Chinese medicine is mainly used in the pathological state, the establishment of PK-PD link model of hypotensive ingredients in Eucommia ulmoides under pathological state will be essential. Importantly, we have found that the exposure of Pinoresinol diglucoside and geniposidic acid with the hypotensive effect contained in Eucommia ulmoides in spontaneously hypertensive rats (SHR) were significantly higher than that of normal rats. Therefore, in the present study, based on spontaneously hypertensive rats and vascular endothelial cell deriving from its aorta and cultured by shear stress, the PK-PD model of hypotensive active ingredients in Eucommia ulmoides will be established from the view of the pathological states and cell pharmacokinetics. The success of this study would further clarify the matter with directly hypotensive effect and provide the scientific basis for the further development of medicinal resources.