铝进入脑细胞可产生严重的中枢神经系统毒性，表现为认知功能障碍等症状。研究表明miRNA与认知功能障碍关系密切,而有关外周血miRNA 的表达变化与铝暴露引起认知功能障碍的研究未见相关报道。本研究拟通过建立铝暴露的大鼠模型，分析外周血淋巴细胞miRNA 的表达和认知功能的关系；同时在小样本铝暴露职业人群中初筛与铝暴露认知功能相关的miRNA；结合上述两者的实验结果选定与铝暴露认知功能相关的差异性miRNA；在铝暴露职业人群扩大样本量对差异性miRNA进行验证；建立AlCl3染毒人神经细胞HCN-2、人神经胶质细胞和淋巴细胞模型，验证差异性miRNA的表达。寻找出预测毒物暴露和疾病发生早期的分子标记物，构建铝暴露的环境-miRNA 交互作用的模型，为职业铝暴露引起的认知功能障碍的早期发现及预防提供理论依据，为研究外周血miRNA 在环境化学污染物引起机体疾病的机制作用提供依据。

As a chronic accumulation neuropoison, aluminium can enter brain cell to induce severe central nervous system toxicity which show recognition functional disturbance. Many researches showed that the recognition functional disturbance is associated with the abnormal expression of miRNA. But there are few reports about the study of miRNA expression profile of peripheral blood in the occupationl workers exposed to aluminium. So in this study we will explore the recognition function and the expression of miRNA in peripheral lymphocytes in the occupationl workers exposed to aluminium in GuangXi mining district, and we will detect the differential miRNA populations in vivo and in vitro. We will study the same variability miRNA in the the occupationl workers and construct the environment- miRNA interaction model in this study,the results of which will support the theory for the early discovery and precaution of the recognition functional disturbance of occupationl workers exposed to aluminium, and which can further support the theory to study the the mechanism of the expression of miRNA of peripheral blood in the diseases caused by the environmental chemical pollutants.