以往研究认为，尿酸钠（MSU）结晶主要通过激活TLRs和NALP3炎性体信号通路，促进IL-1β分泌，导致急性痛风性关节炎（AGA）的发生，但这种理论难以解释部分高尿酸血症患者从不出现痛风、部分痛风急性发作者血尿酸并不增高的现象，提示痛风发病可能存在其他致病信号。.最新研究表明，P2X7R信号通路在GA发病中起着重要作用，可能作为防治GA的新靶点。前期研究表明，清热通络方有较好的疗效，能调控TLRs和NALP3炎性体信号通路，抑制IL-1β释放，而发挥治疗AGA的作用。本研究在此基础上，提出清热通络方可能介导P2X7R信号通路而发挥抗GA的科学假说。以MSU诱导的AGA大鼠、AGA患者及体外培养的AGA患者PBMCs模型为研究对象，采用免疫组化、流式细胞术、PCR等技术，探讨P2X7R信号通路在GA发病中的作用及清热通络方对其的影响，揭示该方防治GA的机制，为其治疗GA提供新的理论依据。

Previous studies suggest that the occurrence of acute gouty arthritis (AGA) is mainly caused by the inflammatory cytokines interleukin-1beta （IL-1β） produced through monosodium urate（MSU）crystallization activating TLRs and NALP3 inflammatory signaling pathways.But the theory of gout disease is difficult to explain some clinical phenomena.For example,some patients with hyperuricemia never have got gout attacks,and serum uric acid of some patients with acute gouty arthritis is not increased.This suggests there may be other pathogenic signals in gout attacks..The latest research shows that P2X7R signaling pathway plays an extremely important role in the pathogenesis of gouty arthritis（GA）,and may be as a new target for the prevention and treatment of GA. Previous research has suggested that Qingre Tongluo Recipe has good clinical curative effect in acute gout arthritis (AGA).The mechanism of Qingre Tongluo Recipe in the treatment of gouty arthritis may be control the release of IL-1β by regulating the TLRs and NALP3 inflammatory signaling pathways.According to the research results,we put forward a scientific hypothesis on Qingre Tongluo Recipe which may prevent gout by mediating P2X7R signaling pathway.In order to reveal some molecular mechanism of Qingre Tongluo Recipe and provide the new theoretical basis for its treatments in gout,Some technologies,such as immunohistochemistry,flow cytometry（FCM）,reverse transcription-polymerase chain reaction（RT-PCR）, western-blotting ,enzyme linked immunosorbent assay（ELISA）and so on,would be adopted to study P2X7R signaling pathway in the pathogenesis of GA,and regulation function of Qingre Tongluo Recipe on rats induced monosodium urate (MSU),patients with acute gouty arthritis(AGA) and PBMCs of the patient in vitro culture.