骨关节炎（OA）是中老年人最常见的退变性骨关节病，已成为全球公共卫生关注的热点。最新研究表明Wnt/β-catenin信号通路在OA发生、发展中起着极其重要的作用，它与软骨和骨的形成和发展关系密切，可作为治疗OA的新靶点。前期研究表明，益气养血方对OA（气血两虚证）有较好的疗效，能调控Wnt/β-catenin信号通路相关下游靶基因X型胶原、蛋白聚糖的表达，促进软骨细胞增殖、分化，有防治关节软骨退变的作用。本研究在此基础上，提出益气养血方可能介导Wnt/β-catenin信号通路调控软骨代谢、软骨细胞增殖、分化与凋亡而发挥抗OA软骨退变的科学假说。以自发性STR/ort小鼠OA模型及体外培养的小鼠退变软骨细胞模型为研究对象，采用组织病理学、细胞学、分子生物学等方法，探讨益气养血方对Wnt/β-catenin信号通路的调控作用，揭示该方抗OA软骨退变的部分分子机制，为其治疗OA提供理论依据。

Osteoarthritis (OA) is a most common degenerative osteoarthropathy in middleaged and elderly people which has become the focus of global public health.The latest research shows that the Wnt/beta-catenin signaling pathway plays an extremely important role in the pathogenesis of osteoarthritis,which has close relationship with the formation and differentiation of cartilage and bone,and may be as a new target for the treatment of osteoarthritis.Previous research has suggested that Yiqi Yangxue Recipe has good clinical curative effect in osteoarthritis（Deficiency of Qi and Blood）.It can promote the proliferation and differentiation of cartilage cells,and can prevent the degeneration of articular cartilage by regulating the expression of related downstream target genes(COL-10 and Aggrecan) in wnt/beta-catenin signaling pathway.According to the research results,we put forward a scientific hypothesis on Yiqi Yangxue Recipe which may prevent osteoarthritis cartilage degenerated through regulating cartilage metabolism,chondrocyte proliferation,differentiation and apoptosis by mediating Wnt/beta-catenin signaling pathways.In order to reveal some molecular mechanism of Yiqi Yangxue Recipe and provide the theoretical basis for its treatments in osteoarthritis,we will study the regulation of Yiqi Yangxue Recipe to Wnt/beta-catenin signaling pathways on the spontaneous STR/ort mice osteoarth ritis model and the mice degenerated chondrocyte in vitro culture by using tissue pathology,cytobiology, molecular biology detecting techniques and so on.