Sialin调控涎腺再生的作用和机制

Sialin为唾液酸及硝酸盐转运蛋白，在上皮和神经系统发育中起到重要的作用。上皮组织中的Sialin可以调节上皮组织周围神经胶质细胞的生长，从而调控外周植物神经纤维的分布。涎腺再生过程中，植物神经可以调控上皮的生长、分化和成熟，失神经支配涎腺无法再生。涎腺上皮组织中的Sialin在涎腺再生中作用和机制还不清楚。通过基因静默、突变基因转染、下颌下腺导管转导Sialin突变基因制造Sialin功能缺陷涎腺上皮细胞和组织，以小鼠涎腺再生模型和涎腺放射损伤模型为研究对象。通过组织学、qPCR、confocal、Western Blot、高效液相色谱等技术，检测涎腺上皮Sialin功能缺陷对涎腺上皮唾液酸转运和代谢、涎腺上皮再生以及神经支配的影响，从而揭示涎腺上皮Sialin在涎腺再生的作用和机制。进一步通过补充唾液酸前体预防小鼠涎腺放射损伤。

Sialin, a transporter of sialic acid and nitrate, possesses the vital role on the development of epithelium and nerve. Sialin in the epithelium could mediate the location of peripheral autonomic nerve by controlling the growth of glial cells around the epithelium. During the regeneration of salivary glands, the peripheral autonomic nerve could maintain and mediate the growth, differentiation, and maturation of salivary epithelium, and the denervation of salivary glands causes it lose the ability of regeneration. The role and mechanism of Sialin in salivary epithelium on the salivary gland regeneration still are not uncovered. To reveal the role and mechanism of Sialin on the regeneration of salivary glands, the relationship of salivary epithelium and autonomic innervations is focused. The defeated Sialin in salivary cells and tissues is produced by knockdown and mutant gene transfection in vitro, and mutant gene transduction in vivo. Ligation and deligation of submandibular gland main ducts causes a model of salivary gland regeneration. Radiation causes a model of salivary gland damage. To evaluated the conditions that defeated Sialin leads the changes of sialic acid transporting and metabolism, the regeneration of salivary glands, and autonomic innervations, histological stain, qPCR, confocal, Western Blot, high performance liquid chromatography, etc. are utilized. Furthermore, a preclinical study is done in which the precursor of sialic acid is administrated pre-radiation to prevent salivary glands from damage caused by radiation.