古遗址中蚕丝蛋白微痕迹免疫检测研究

本项目通过研究不同降解阶段的蚕丝蛋白微痕迹的一级结构和空间结构的变化，建立蚕丝蛋白降解程度和劣化状态的量化关系，探索和认识蚕丝蛋白的降解规律。通过分析古遗址中蚕丝蛋白微痕迹的分子结构和氨基酸序列，将具有特定序列的多肽片段选定为蚕丝蛋白的分子标志物，通过动物免疫的方法制备得到可对古代丝绸进行专一性识别的特异性抗体。利用免疫学的原理和方法，结合考古学信息，构建基于免疫技术的丝绸文物微痕迹检测体系，为古遗址中已经化作无形的丝绸微痕迹提供一种敏感、特异、快捷的辨识方法，同时为研究丝绸的起源和传播提供新的研究思路和科学证据。

To reveal the relationship between the structures and degradation stage , and to further explore the nature of silk fibroin degradation, this program is dedicated to study the changes of primary structure and spatial configuration at the molecular level for the ancient silks micro-traces. By analysing the amino acid sequences of ancient silk fibroin, several diagnostic sequences will be selected as molecular markers for silk fibroin and specific antibodies against fibroin will be prepared in rabbits through peptide synthesis and carrier-protein coupling. And then, the identification methods of ancient silk traces based on immunological techniques will be established. These methods not only have the potential to become powerful tools for the rapid, sensitive and specific detection of invisible silk residues in the archaeological field, but also provide a new protocol for identifying the origin and transmission of ancient silks.