微丝菌导致的污泥丝状膨胀是国内外该领域关注的热点问题，对其生长代谢机制与微丝菌过度繁殖关系方面已有很多研究报道,但到目前为止还没能找到及时抑制其膨胀的有效方法。本项目采用设计制备的微丝菌及体内标记物的靶向分子影像探针，观察微丝菌在污泥原位条件下对长链脂肪酸（LCFAs）的吸收、运移、分解代谢过程，探索微丝菌代谢过程中胞内聚合物、聚磷盐的数量变化、赋存速率和空间分布规律，研究其定性定量荧光信息与微丝菌的代谢作用机制、能量关系及污泥膨胀的相关性并建立数学模型；探索微丝菌生命代谢特征与污泥膨胀过程的相关性,加深对微丝菌导致污泥膨胀关键因素的理解，探求微丝菌导致污泥膨胀的抑制和控制因素。投加LCFAs表面修饰的微硅粉，研究污泥系统中微丝菌的聚集形态及对污泥沉降性能的影响。其科学意义在于揭示微丝菌在污泥系统中的代谢过程对污泥膨胀状态的决定关系，这对抑制和控制微丝菌污泥膨胀具有重要意义。

Nowadays, sludge bulking caused by Microthrix parvicella has been the hot research point in environmental field. Plenty of research has been focused on the reason of the overgrowth of M. parvicella as well as its growth metabolism mechanism. However researchers havn’t found an efficient method to inhibit the bulking until now. In our study, we intend to design and synthesize molecular imaging probes, which can specifically target to M. parvicella and other markers in the bacteria. By observing the absorbtion, transfer and catabolism process of long chain fatty acids (LCFAs) in the presence of M. parvicella in sludge, we will investigate the amount change, velocity and spatial distribution regularities of the polyphosphate and polymer in cells. A mathematical model will be built based on the relationship between the fluorescence information and the metabolism of M. parvicella, enengy, sludge bulking, etc. Furthermore, we will explore the the relationship between the metabolism of M. parvicella and sludge bulking, deeply understand the key factor of sludge bulking caused by M. parvicella, and study the control condition to inhibit the bulking. By adding LCFAs modified micro silica fume into the sludge system, finally we will investigate the aggregation morphology of M. parvicella and the effect of silica fume on sludge settleability. The scientific significance of the study is to reveal how the metabolism of M. parvicella can determine the sludge bulking, which is of great importance to inhibit and control the sludge bulking.